The effect of antibiotics and the gut flora on the immune system.
Completed
- Conditions
- sepsis, gut flora depletion, systemic immune response
- Registration Number
- NL-OMON27808
- Lead Sponsor
- Academisch Medisch Centrum, Amsterdam
- Brief Summary
/A
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 12
Inclusion Criteria
1. Healthy;
2. Male between 18 and 35 years of age;
Exclusion Criteria
1. Major illness in the past 3 months or significant chronic medical illness;
2. History of any type of malignancy;
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Side effects, laboratory measurements, functional assays and microbiota composition.<br><br /><br /><br>1. Side effects will be registered using a graded scale;<br /><br>2. A HITchip (16S rRNA) analysis to determine microbiome composition;<br /><br>3. Laboratory measurements: include inflammatory markers, neutrophil activation and -degranulation;<br /><br>4. Functional assays: ex vivo stimulation of blood cells with nosocomial pathogens.
- Secondary Outcome Measures
Name Time Method