Comparison of the Genotoxicity of Desflurane and Propofol

Not Applicable

Completed

• Conditions: DNA Damage; Anesthesia
• Interventions: Drug: Anesthetics, General Desflurane Maintenance; Drug: Anesthetics, General Propofol Maintenance

• Registration Number: NCT05185167
• Lead Sponsor: Muğla Sıtkı Koçman University

Brief Summary

The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.

Detailed Description

It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.

Study Timeline

• Study First Submitted: 2021-12-06
• Study First Submitted QC: 2022-01-06
• Study First Posted: 2022-01-11
• Study Start: 2022-01-17
• Status Verified: 2022-03
• Primary Completion: 2022-03-15
• Study Completion: 2022-03-20
• Last Update Submitted: 2022-03-29
• Last Update Posted: 2022-03-31

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

1. Ability to understand and willingness to sign an informed consent form
2. Elective lumbar spinal surgery that will take at least 120 minutes,
3. American Society of Anesthesiologists (ASA) physical status I and II

Exclusion Criteria

1. Patients with ASA physical status III or IV disease
2. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders)
3. Smoking or alcohol consumption
4. Recently received radiation, chemotherapy
5. Malignancy
6. Patients who will receive blood transfusion during the operation
7. Users of antioxidant supplements
8. Patients who have received general anesthesia in the last three months
9. Patients with known occupational exposures (operating room personnel, chemical plant worker

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SEQUENTIAL

Arm && Interventions

Group	Intervention	Description
Desflurane Group	Anesthetics, General Desflurane Maintenance	Desflurane 1,0 minimum alveolar concentration will be administered during anesthesia maintenance
Propofol Group	Anesthetics, General Propofol Maintenance	Propofol 6 mg//kg/hour will be administered during anesthesia maintenance

Primary Outcome Measures

Name	Time	Method
Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	postoperative 5th day	The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.
Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	postoperative 5th day	The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.
Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	postoperative 5th day	The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.

Trial Locations

Locations (1)

Mugla Sitki Kocman University; 🇹🇷 Mugla, Turkey