Tissue Sectioning by Electro-Dissociation

Withdrawn

• Conditions: Fresh Unfrozen Tissues; Thin Sections; Fresh Unfixed Tissues

• Registration Number: NCT01054612
• Lead Sponsor: University of Arkansas

Brief Summary

Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Detailed Description

Not available

Study Timeline

• Study Start: 2002-03
• Study First Submitted: 2007-12-21
• Primary Completion: 2010-01
• Study Completion: 2010-01
• Study First Submitted QC: 2010-01-20
• Study First Posted: 2010-01-22
• Status Verified: 2015-03
• Last Update Submitted: 2015-03-03
• Last Update Posted: 2015-03-04

Recruitment & Eligibility

• Status: WITHDRAWN
• Sex: All
• Target Recruitment: Not specified

Inclusion Criteria

• Fresh Tissue
• Unfrozen Tissue
• Unfixed Tissue

Exclusion Criteria

• Unfresh Tissue
• Frozen Tissue
• Fixed Tissue

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified