Blood Pressure and Central Haemodynamics in Healthy Adults Stratified by MTHFR Genotype
- Conditions
- Blood Pressure
- Registration Number
- NCT04948086
- Lead Sponsor
- University of Ulster
- Brief Summary
Approximately 12% of the world's population have a have a common C677T polymorphism in the gene encoding the folate metabolising enzyme, methylenetetrahydrofolate reductase (MTHFR). Homozygosity for the polymorphism (TT genotype) appears to result in an increased requirement for the B-vitamins folic acid and riboflavin and more importantly results in an increased risk of developing high blood pressure (BP). Previous work from our Centre has demonstrated significantly higher BP in those with the TT genotype. This work has been conducted in cohorts with premature cardiovascular disease (CVD) and hypertension without overt CVD, but the effect in younger, healthier individuals is unexplored. To date our studies have also focused on BP as the primary outcome, but newer markers of vascular health including central pressure and hemodynamics have emerged as superior prognostic indicators of CVD. The effect of the TT genotype on these measures is thus an important area for investigation and may help us understand the mechanism linking the genotype with BP, which is currently unknown. As adults with the TT genotype appear to have increased requirements for riboflavin and folic acid, and BP in TT adults appears to be riboflavin dependent, the influence of these vitamins on central measures is an area for consideration. Study Design This is an observational study investigating the blood pressure profiles of healthy adults aged 18-65 years, stratified by MTHFR genotype. Apparently healthy adults will be recruited from workplaces and the general community across Northern Ireland and screened for the polymorphism via buccal swab. Those with the TT genotype and a similar number of non-TT (i.e. CC/CT) genotype individuals will be contacted and asked to come to attend a one-off appointment. Brachial BP will be assessed by an electronic BP monitor, central BP and central haemodynamics (augmentation index, augmentation pressure and pulse wave velocity) will be assessed by SphygmoCor XCEL. In addition, anthropometric measurements, health and lifestyle information and a blood sample will be obtained. Data will be statistically analysed using SPSS software to if determine differences between gentoype groups exist.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 498
- Pre-screened for MTHFR 677TT genotype
- Aged at least 18 years old
- Younger than 18 years
- Pregnant
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Blood pressure One day, one measurement Office blood pressure
- Secondary Outcome Measures
Name Time Method Pulse wave velocity One day, one measurement Measured using SphygmoCor device
Brachial blood pressure One day, one measurement Measured using SphygmoCor device
Central blood pressure One day, one measurement Measured using SphygmoCor device
One-carbon metabolite status One day, one measurement Measured by GCMS-MS
Pulse wave analysis One day, one measurement Measured using SphygmoCor device
Folate status One day, one measurement Measured by microbiological assay
PLP status One day, one measurement Measured by HPLC
Homocysteine status One day, one measurement Measured by GCMS
Riboflavin status One day, one measurement Measured by by EGRac
Trial Locations
- Locations (1)
Human Intervention Studies Unit, Ulster University
🇬🇧Coleraine, Co.Londonderry, United Kingdom