Exercise-induced Changes in Exosomes
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Enrollment
- 12
- Locations
- 1
- Primary Endpoint
- Collagen content of ligament constructs
Study Overview
Brief Summary
The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Treatment
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Young healthy adults (18-35 y)
- •Experience with exercise (>1y)
Exclusion Criteria
- •Pregnancy
- •Receiving any medication that may interfere with the study outcomes
Arms & Interventions
Exercise trial
Participants will perform a bout of exercise. In addition, a baseline and post-exercise blood sample will be drawn.
Intervention: Resistance Exercise (Procedure)
Outcomes
Primary Outcomes
Collagen content of ligament constructs
Time Frame: 15 min post-exercise
Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay.
Secondary Outcomes
- Ultimate tensile stress and modulus of ligament constructs(15 min post-exercise)
- Exosomal RNA(15 min post-exercise)