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The Effect of Pomegranate on Aging and Inflammation of the Skin

Not Applicable
Completed
Conditions
Skin Photoaging, Inflammation and Skin Pathogenic Bacteria
Interventions
Dietary Supplement: Pomegranate Extract
Dietary Supplement: Pomegranate Juice
Dietary Supplement: Placebo
Registration Number
NCT02258776
Lead Sponsor
University of California, Los Angeles
Brief Summary

The purpose of this study is to determine the efficacy of pomegranate extract and pomegranate juice on skin inflammation and aging. The information gained from this study may lead to the development of a pomegranate product that can decrease the effects of aging, inflammation and harmful bacteria on the skin.

In this study, two pomegranate products (extract and juice) will be compared with a placebo, a study product that looks like pomegranate extract, but contains no active ingredients.

Detailed Description

Pomegranate (Punica granatum L.) is grown commercially in the Near East, India, Spain, Israel and the United States (California) where it is of significant economic importance1. Pomegranate fruits and products, including juice, tea, wine and extracts are widely consumed and recognized for their health benefits. Pomegranate (Punica granatum L.) fruit possesses strong antioxidant, anti-inflammatory and antiproliferative properties. In recent years, most health advantages of pomegranate have been attributed to the presence of ellagitannins, mainly punicalagins and ellagic acid. Punicalagin is unique to pomegranate and is part of a family of ellagitannins. The ellagitannins are hydrolyzed to ellagic acid in the gut, and further metabolized by the colon microflora to form urolith A and B. Investigations using pure bacterial cultures have shown that pomegranate by-products and punicalagins significantly inhibited the growth of pathogenic Escherichia coli, Pseudomonas aeruginosa, clostridia and Staphylococcus aureus.

Oral feeding of pomegranate fruit extract to mice afforded protection to mouse skin against the adverse effects of ultraviolet-B (UVB) radiation by modulating UVB-induced signaling pathways.5 Hydroalcoholic extract based-ointment from pomegranate was reported improving wound healing in vivo. Pomegranate ellagitannins have been demonstrated to have antimicrobial activity. Punicalagin is unique to pomegranate and is part of a family of ellagitannins. The ellagitannins are hydrolyzed to ellagic acid in the gut, and further metabolized by the colon microflora to form urolith A and B. Investigations using pure bacterial cultures have shown that pomegranate by-products and punicalagins significantly inhibited the growth of pathogenic Escherichia coli, Pseudomonas aeruginosa, clostridia and Staphylococcus aureus.

Pomegranate Extract (POMx) is made from pomegranate fruit as a byproduct of pomegranate juice (PJ) production. A second pressing of the fruit liberates a complex mixture of hydrolysable polyphenolic compounds normally ingested with pomegranate juice, and these are purified by spray drying. POMx powder is encapsulated for oral administration, with each capsule containing 1,000 mg of pomegranate polyphenols. The present study will determine whether a pomegranate product (POMx or PJ) can decrease skin photoaging, inflammation and skin pathogenic bacteria.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
75
Inclusion Criteria
  1. Age 30-45 years or older, female and any racial/ethnic group
  2. Subjects must understand and sign the informed consent prior to participation
  3. Subjects must be in generally good health
  4. Subjects must be able and willing to comply with the requirements of the protocol.
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Exclusion Criteria
  1. Oral or topical retinoid use within twelve months of entry into the study
  2. Non-compliant subjects
  3. Taking any antibiotics or other medication or dietary supplements regularly
  4. Subjects with a significant medical history or concurrent condition that the investigator(s) feel is not safe for the study
  5. Subjects who cannot avoid excessive exposure to either natural or artificial sunlight.
  6. Allergic to pomegranate products
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Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Pomegranate ExtractPomegranate Extract15 healthy subjects, ages 30-45 years who meet all the eligibility criteria in the screening phase of the study will be assigned to the Pomx arm of the study to evaluate the clinical efficacy of pomegranate extract on skin inflammation and aging. Subjects will be asked to take Pomx 1/day for 12 weeks.
Pomegranate JuicePomegranate Juice15 healthy subjects, ages 30-45 years who meet all the eligibility criteria in the screening phase of the study will be assigned to the Pomx arm of the study to evaluate the clinical efficacy of pomegranate juice on skin inflammation and aging. Subjects will be asked to take Pomx 1/day for 12 weeks.
PlaceboPlacebo15 healthy subjects, ages 30-45 years who meet all the eligibility criteria in the screening phase of the study will be assigned to the Pomx arm of the study to evaluate the clinical efficacy of placebo on skin inflammation and aging. Subjects will be asked to take Pomx 1/day for 12 weeks.
Primary Outcome Measures
NameTimeMethod
Change in inflammatory markers between pomegranate extract and pomegranate juice versus placebo.Baseline and 12 weeks

Utilization of a two-sample t-test to compare the change in between the three groups

Secondary Outcome Measures
NameTimeMethod
Linear mixed effects models to evaluate effect of treatment of time trends and demographic covariatesBaseline and 12 weeks

Correlation between outcomes measures (e.g. between acne lesion count and skin microbiota) with mixed effects models

Trial Locations

Locations (1)

UCLA Center for Human Nutrition

🇺🇸

Los Angeles, California, United States

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