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Intradermal naked DNA vaccination for mounting tumor-specific immunity in stage IV melanoma patients: a phase I clinical study

Completed
Conditions
melanoma
skin cancer
10027476
Registration Number
NL-OMON35508
Lead Sponsor
KI-AV
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
Not specified
Target Recruitment
15
Inclusion Criteria

• Age above 18 years
• Performance score: WHO 0 or 1
• Life expectancy of >= 3 months
• Histologically or cytologically proven metastatic melanoma.
• Expression of MART-1.
• HLA-A*0201 positive.
• Evaluable disease.
• Disease progression after chemotherapy-based treatment.
• Adequate bone marrow (WBC > 3.0/nL, platelets > 100/nL), renal function (creatinine clearance > 40 mL/min, and liver function (bilirubin < 1.5 x ULN, normal blood coagulation)
• Willing and able to undergo the planned study procedures

Exclusion Criteria

• Previous MART-1-specific immunotherapy.
• Patients with severe cardiac, respiratory, or metabolic disease.
• Symptomatic brain metastases.
• Use of systemic steroids or other immunosuppressive drugs.
• Use of oral anticoagulant drugs.
• Other cancers, except basal cell carcinomas or cervical CIS.
• Severe infections requiring antibiotics.
• Lactation or pregnancy
• Not willing to take adequate contraceptive measures.

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
<p>Main study parameters/endpoints:<br /><br>1.) Dose limiting toxicity of intradermal DNA vaccination using a plasmid<br /><br>encoding tetanus toxin fragment c and an immunodominant HLA-A2 binding MART-1<br /><br>peptide.<br /><br>2.) Unacceptable toxicity of the use of a new device for intradermal delivery<br /><br>of plasmid DNA. </p><br>
Secondary Outcome Measures
NameTimeMethod
<p>1.) Immunological response against MART-1 in blood and biopsy material<br /><br>2.) Objective clinical responses according RECIST</p><br>
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