E. Faecalis Prevalence in Primary and Secondary Endodontic Infections: a Pre-post Microbial Analysis Following Chemo-mechanical Preparation

Completed

• Conditions: Apical Periodontitis
• Interventions: Procedure: Root Canal Treatment

• Registration Number: NCT04637659
• Lead Sponsor: University of Siena

Brief Summary

Post-treatment apical periodontitis is a fairly prevalent condition frequently caused by a persistent endodontic infection due to failure of the endodontic treatment. Microbial species in treated or untreated root canals were found to be different, with the former being less diverse and mainly characterized by Gram positive, facultative anaerobes bacteria than the latter. Enterococcus faecalis is the most frequently detected species in root-filled teeth in many studies (Zhang et al., 2015). In fact, E. Faecalis retains many virulence factors allowing adhesion to host cells and extracellular matrix, tissue invasion and damage through toxins release, as well as the ability to survive even in harsh environmental conditions. Further studies are needed to clarify E. faecalis prevalence among the different forms of pulpal and periapical lesions as well as its correlation with clinical symptoms.

Detailed Description

Not available

Study Timeline

• Study Start: 2019-06-01
• Primary Completion: 2020-10-01
• Study Completion: 2020-10-10
• Status Verified: 2020-11
• Study First Submitted: 2020-11-13
• Study First Submitted QC: 2020-11-19
• Last Update Submitted: 2020-11-19
• Study First Posted: 2020-11-20
• Last Update Posted: 2020-11-20

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 72

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Necrosis	Root Canal Treatment	Teeth being treated because of pulpal necrosis
Healthy treated teeth	Root Canal Treatment	Teeth being retreated for restorative or prosthodontics reasons
Post-treatment apical periodontitis	Root Canal Treatment	Teeth being retreated due to the presence of an apical lesion
Healthy non-treated teeth	Root Canal Treatment	Healthy teeth being treated for restorative or prosthodontics reasons
Irreversible pulpitis	Root Canal Treatment	Teeth being treated because of a poor pulpal status

Primary Outcome Measures

Name	Time	Method
Prevalence of E.Faecalis in the canal before the treatment for each group	Pre-treatment microbial sampling in the canal was carried out 1 day once arrived at working length with a 10 K-file	E.Faecalis prevalence in the canal before the treatment will be calculated for each group.

Secondary Outcome Measures

Name	Time	Method
Correlation between E.Faecalis presence in the canal before the treatment and the pulpal/periapical status	Pre-treatment microbial sampling in the canal was carried out 1 day once arrived at working length with a 10 K-file	Correlation between E. faecalis presence in the canal before the treatment and the pulpal/periapical status for each group will be calculated through measures of association

Trial Locations

Locations (1)

AOUS; 🇮🇹 Siena, Italy