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Study of Interleukin-2, Interferon-alpha and Bevacizumab in Metastatic Kidney Cancer

Phase 2
Conditions
Metastatic Renal Cell Carcinoma
Interventions
Registration Number
NCT01274273
Lead Sponsor
University of Aarhus
Brief Summary

The purpose of this study is to determine whether interleukin-2, interferon-alpha in combination with bevacizumab are effective in the treatment of metastatic renal cell carcinoma (mRCC).

Detailed Description

Bevacizumab as monotherapy has effect in metastatic renal cell carcinoma (mRCC). Bevacizumab in combination with interferon-alfa (IFN-α) has significant efficacy in mRCC and has been approved by EMA and FDA.

The present study will assess whether the combination of Interleukin-2 (IL-2) and IFN-α with bevacizumab may add efficacy in patients with mRCC with a tolerable safety profile.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
118
Inclusion Criteria
  1. Signed written informed consent
  2. Patient must be willing and able to comply with the protocol.
  3. Age ≥ 18 years.
  4. Histologic og cytologic biopsy proven locally advanced or metastatic renal cell carcinoma, considered non-candidates for curative surgery. Nephrectomy is not mandatory.
  5. Patient with renal cell carcinoma (RCC) with a clear-cell histologic component confirmed by local pathology review.
  6. Females with a negative serum pregnancy test unless childbearing potential can be otherwise excluded
  7. Fertile women of childbearing potential (<2 years after last menstruation) and men must use effective means of contraception
  8. Memorial-Sloan-Kettering-Cancer-Centre favourable- and intermediate prognostic group.
  9. Measurable or non-measurable disease (as per RECIST1.1 criteria)
  10. Karnofsky Performance status of 70% or higher.
  11. Life expectancy greater than 4 months.
  12. The required laboratory values at baseline are as follows:

Haematology:

WCC ≥ 3.0 x 109/L, Platelet count ≥ 100 x 109/L, Haemoglobin ≥ 6.2 mmol/l, (INR) ≤ 1.5, APTT ≤ 1.5 x ULN

Biochemistry:

Total bilirubin ≤ 1.5 x upper limit of normal (ULN), AST, ALT ≤ 2.5 x ULN in patients without liver metastases, ≤ 5 x ULN in patients with liver metastases, Serum Creatinine ≤ 150 micromol/L

Exclusion Criteria
  1. Prior systemic treatment for metastatic RCC disease
  2. Major surgical procedure, open surgical biopsy, or significant traumatic injury within 28 days prior to randomization.
  3. Serious non-healing wound, ulcer or bone fracture.
  4. Evidence of current central nervous system (CNS) metastases or spinal cord compression. Patient must undergo an MRI or CT scan of the brain (with contrast, if possible) within 28 days prior to randomization.
  5. Seizure(s) not controlled with standard medical therapy.
  6. Dipstick urine test of protein ≥ 2+.
  7. Other malignancies within 5 years prior to randomization (other than curatively treated basal cell carcinoma of the skin and/or in situ carcinoma of the cervix).
  8. Evidence of bleeding diathesis or coagulopathy.
  9. Ongoing or recent (within 10 days prior to study treatment start) need for full therapeutic dose of oral anticoagulants or chronic daily treatment with aspirin. Low molecular weight heparin are allowed
  10. Uncontrolled hypertension (≥ 160 mm Hg systolic and/or ≥ 100 mm Hg diastolic) while receiving chronic medication.
  11. Clinically significant (i.e. active) cardiovascular disease, for example cerebrovascular accidents (≤ 6 months before randomisation), myocardial infarction (≤ 6 months before randomisation), unstable angina, New York Heart Association (NYHA) grade II or greater congestive heart failure, or serious cardiac arrhythmia requiring medication.
  12. Recent (within the 30 days prior to randomization) treatment with another investigational drug or participation in another investigational study.
  13. Chronic treatment with corticosteroids (dose of ≥ 10 mg/day methylprednisolone equivalent), excluding inhaled steroids.
  14. History or presence of other disease, metabolic dysfunction, physical examination finding, or clinical laboratory finding giving reasonable suspicion of a disease or condition that contraindicates use of an investigational drug or patient at high risk from treatment complications.
  15. Known hypersensitivity to interleukin-2, Interferon, alfa or bevacizumab.

Serial blood test, serial tumor biopsies and serial dynamic contrast-enhanced imaging will be obtained as part of a translational research program integrated in the clinical trial.

Part(s) of the translational research program may be omitted in the individual patient due to practical, technical or safety reasons, without having consequences for participating in the additional translational research investigations or the clinical part of the study.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Interleukin-2 and interferon-alfaInterleukin-2-
Interleukin-2 and interferon-alfaInterferon Alfa-2b-
Interleukin-2, interferon, bevacizumabBevacizumab-
Primary Outcome Measures
NameTimeMethod
Progression free survival, PFSThis is defined as the time between date of randomisation and the first date of documented disease progression or date of death due to any cause.
Secondary Outcome Measures
NameTimeMethod
Frequency of surgical resection of residual diseasesee below

This is calculated as number of patients having surgical resection of residual disease compared with the total number of treated patients.

Overall survival, (OS)Overall survival is defined as the time between date of randomisation and the date of death due to any cause.
Time to treatment failure, (TTTF)see below

Time to treatment failure is defined as time between date of randomisation and date of insufficient therapeutic response (including disease progression), death, withdrawal of treatment due to adverse events or laboratory abnormality, or withdrawn informed consent.

Tolerabilitysee below

Toxicity is recorded according to CTCAE v3.0

To explore the immunomodulatory effect of therapy in serial blood samples and serial tumor core biopsies and to correlate these biomarkers with outcomesee below

Blood tests and core biopsies from accessible tumor lesions will be obtained at baseline, after cycle 1 and at PD.

Blood analyses will include assessment of dendritic cells, FoxP3+ regulatory T-cells, NK-cells, T-subsets, neutrophils, monocytes, cytotoxic activity and antibody-dependent cellular cytotoxicity (ADCC).

Tumor analyses will include assessment of intratumoral immune cells, markers related to HIF accumulation and CD34+ microvessel density.

Response rate, RROverall response rate as assessed by the RECIST 1.1 criteria. An overall response is defined as a confirmed complete response (CR) or confirmed partial response (PR).
Duration of responseDuration of response is defined as the time between the date a response (CR or PR) was first seen until date of progression.
Time to progression, (TTP)Time to progression is defined as time between date of randomisation and date of documented progression.
Frequency of no evidence of disease (NED)see below

This is calculated as the total number of patients having no evidence of disease as a result of CR to treatment, or as a result of PR/SD to treatment followed by surgical resection of residual disease, compared with the total number of treated patients.

To assess dynamic contrast-enhanced imaging as a potential biomarker.see below

Dynamic contrast-enhanced imaging (CT, MRI, and US) will be obtained at baseline, week 5 and at routine tumor assessments, if appropriate, for estimation of tumor blood perfusion change.

An exploratory analysis to identify any potential relationship between each of these assessments and outcome (progression free survival, survival, time to progression, response rate and safety) will be performed.

Trial Locations

Locations (2)

Aarhus University Hospital

🇩🇰

Aarhus, Denmark

Herlev University Hospital

🇩🇰

Herlev, Denmark

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