Eccentric Exercise and Oxidative Stress

Not Applicable

Completed

• Conditions: Aging; Muscle Damage; Oxidative Stress
• Interventions: Other: Exercise

• Registration Number: NCT01728675
• Lead Sponsor: European University Cyprus

Brief Summary

The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.

Detailed Description

Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.

Study Timeline

• Study Start: 2012-05
• Primary Completion: 2012-07
• Study Completion: 2012-07
• Status Verified: 2012-11
• Study First Submitted: 2012-11-06
• Study First Submitted QC: 2012-11-13
• Last Update Submitted: 2012-11-13
• Study First Posted: 2012-11-20
• Last Update Posted: 2012-11-20

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 20

Inclusion Criteria

• subject provides written informed consent
• physiological health profile
• stable at their anthropometric characteristics for at least the last year

Exclusion Criteria

• muscle disease
• prior history of musculoskeletal injury to the lower limbs
• smokers
• consumed any nutritional supplement the last 3 months
• performed intense eccentric exercise the last 6 months
• non Caucasian

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Young group	Exercise	Participants will underwent two isokinetic eccentric exercise sessions
Elderly group	Exercise	Participants will underwent two isokinetic eccentric exercise sessions

Primary Outcome Measures

Name	Time	Method
Delayed onset muscle soreness, DOMS (scale 1-10)	Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Muscle torque (torque)	Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Range of motion, ROM (degrees)	Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Creatine kinase, CK (activity IU)	Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Secondary Outcome Measures

Name	Time	Method
F2-isoprostanes (pg/mg creatinine)	Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Protein carbonyls (nmol/mg protein)	Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Glutathione, GSH (μmol/g Hb)	Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Catalase (U/g Hb)	Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Superoxide dismutase, SOD (U/g Hb)	Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Glutathione peroxidase, GPx (U/g Hb)	Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)	Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Uric acid (mM)	Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Albumin (μM)	Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Bilirubin (μM)	Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise

Trial Locations

Locations (1)

European University Cyprus; 🇨🇾 Nicosia, Cyprus