Eccentric Cycling Exercise on Mitochondrial Function of Lymphocyte

Not Applicable

Completed

• Conditions: Eccentric Exercise Training
• Interventions: Behavioral: Eccentric cycling training; Behavioral: Concentric cycling training

• Registration Number: NCT06576804
• Lead Sponsor: Chang Gung Memorial Hospital

Brief Summary

Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established.

Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.

Detailed Description

Eccentric exercise training (EET) increases physical performance while having lower metabolic demand than concentric exercise training (CET).

Mitochondria of lymphocytes are essential for cell proliferation, death, and differentiation, and play a critical role in establishing lymphocyte phenotypes and their functions.

Whether EET influences lymphocyte bioenergetic efficiency remains unclear. The study was to investigate the effects of ECE and CCE regimens on adaptive immune functions and mitochondrial bioenergetics of T lymphocytes in sedentary males.

Study Timeline

• Study Start: 2021-01-01
• Primary Completion: 2022-01-01
• Study Completion: 2022-08-01
• Status Verified: 2024-08
• Study First Submitted: 2024-08-20
• Study First Submitted QC: 2024-08-27
• Last Update Submitted: 2024-08-27
• Study First Posted: 2024-08-29
• Last Update Posted: 2024-08-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 33

Inclusion Criteria

1. Sedentary lifestyle.
2. Nonsmokers, nonusers of medications/vitamins.
3. No any cardiopulmonary/hematological risks.

Exclusion Criteria

1. Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
2. Have retabolic or musculoskeletal disease.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Eccentric cycling training	Eccentric cycling training	Moderate to high intensity cycling training in eccentric type
Concentric cycling training	Concentric cycling training	Moderate to high intensity cycling training in concentric type (intensity matched the eccentric training)

Primary Outcome Measures

Name	Time	Method
Mitochondrial function of lymphocyte	12 weeks	Evaluate bioenergetic health index (BHI) of mitochondria in lymphocyte by using a high resolution respirometer.

Secondary Outcome Measures

Name	Time	Method
Cardiopulmonary fitness	12 weeks	The graded exercise test (GXT) on a bicycle ergometer was performed. The GXT comprised 2 min of unloaded pedaling followed by a continuous increase in the work rate of 30 watt per 3-minute until exhaustion (i.e., progressive exercise to maximal O2 consumption)

Trial Locations

Locations (1)

Chang Gung University; 🇨🇳 Taoyuan, Taiwan