The Effect of Eccentric Exercise on Oxidative Stress in the Elderly
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Aging
- Sponsor
- European University Cyprus
- Enrollment
- 20
- Locations
- 1
- Primary Endpoint
- Delayed onset muscle soreness, DOMS (scale 1-10)
- Status
- Completed
- Last Updated
- 13 years ago
Overview
Brief Summary
The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.
Detailed Description
Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.
Investigators
Dr. Anastasios Theodorou
Researcher
European University Cyprus
Eligibility Criteria
Inclusion Criteria
- •subject provides written informed consent
- •physiological health profile
- •stable at their anthropometric characteristics for at least the last year
Exclusion Criteria
- •muscle disease
- •prior history of musculoskeletal injury to the lower limbs
- •consumed any nutritional supplement the last 3 months
- •performed intense eccentric exercise the last 6 months
- •non Caucasian
Outcomes
Primary Outcomes
Delayed onset muscle soreness, DOMS (scale 1-10)
Time Frame: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Muscle torque (torque)
Time Frame: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Range of motion, ROM (degrees)
Time Frame: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Creatine kinase, CK (activity IU)
Time Frame: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Secondary Outcomes
- F2-isoprostanes (pg/mg creatinine)(Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Protein carbonyls (nmol/mg protein)(Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Glutathione, GSH (μmol/g Hb)(Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Catalase (U/g Hb)(Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Superoxide dismutase, SOD (U/g Hb)(Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Glutathione peroxidase, GPx (U/g Hb)(Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)(Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Uric acid (mM)(Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Albumin (μM)(Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)
- Bilirubin (μM)(Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise)