The Effect of L-arginine and L-citrulline Supplementation on Endurance Performance and Mitochondrial Respiration
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 18
- 试验地点
- 2
- 主要终点
- Change in Endurance performance on a cycle ergometer between L-arginine supplementation and placebo supplementation
研究概览
简要总结
The objective of this trial is to determine the effects of dietary supplementation with the amino acids, L-arginine and L-citrulline, on endurance performance and mitochondrial respiration in healthy men compared to supplementation with L-arginine alone and a placebo supplement.
详细描述
This study is a randomized, double-blind, cross over trial of L-arginine and L-citrulline supplementation, L-arginine supplementation alone and placebo supplementation in healthy men. The intervention period will be 8-11 weeks for each participant and a minimum of 15 individuals will be recruited and enrolled to complete the trial. Subjects will complete the experimental protocol described below over 3 individual 10 day supplementation periods, separated by washout periods of approximately 2 weeks. Subjects will ingest corn-starch as a placebo, L-arginine (3g/day) or L-arginine (1.5 g/day) + L-citrulline (1.5g/day). On day 7 of the supplementation period, a skeletal muscle biopsy and blood sample will be obtained from the subjects to investigate mitochondrial function and blood amino acid and nitric oxide levels. On day 10 of the supplementation period, subjects will complete a performance test on a cycle ergometer to investigate their endurance performance. These assessments will be performed to determine the effects of co-ingesting L-arginine and L-citrulline compared to L-arginine alone and placebo administration.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •healthy male, 18-40 years of age
- •recreationally-active
- •healthy BMI (≥18.5 and ≤24.9 kg/m2)
排除标准
- •participating in other studies currently or in the past two months or has an intention to participate in other studies over the period of this study
- •history of gastric, digestive, cardiovascular, renal disease or any other orthopaedic diseases related to motor organs
- •lidocaine allergy
- •use of medication that may affect study measures
- •contraindication to the biopsy procedures
- •on medications that affect the immune system
- •on Aspirin or other drugs known to prolong bleeding
- •user of dietary supplements currently or in the past one month
- •a heavy drinker
结局指标
主要结局
Change in Endurance performance on a cycle ergometer between L-arginine supplementation and placebo supplementation
时间窗: 8-11 weeks
On day 10 of the supplementation periods, subjects will complete a performance test on a cycle ergometer to investigate their endurance performance
Changes of mitochondrial function in skeletal muscle between L-arginine supplementation and placebo supplementation
时间窗: 8-11 weeks
On day 7 of the supplementation periods, a skeletal muscle biopsy sample will be obtained from subjects to investigate mitochondrial function.
Changes of mitochondrial function in skeletal muscle between L-arginine + L-citrulline supplementation and placebo supplementation
时间窗: 8-11 weeks
On day 7 of the supplementation periods, a skeletal muscle biopsy sample will be obtained from subjects to investigate mitochondrial function.
Change in Endurance performance on a cycle ergometer between L-arginine + L-citrulline supplementation and placebo supplementation
时间窗: 8-11 weeks
On day 10 of the supplementation periods, subjects will complete a performance test on a cycle ergometer to investigate their endurance performance
次要结局
- Changes in muscle oxygenation between L-arginine + L-citrulline supplementation and placebo supplementation(8-11 weeks)
- Changes of amino acid and nitric oxide biomarkers levels in plasma between L-arginine supplementation and placebo supplementation(8-11 weeks)
- Changes in subjective feeling during and after exercise between L-arginine + L-citrulline supplementation and placebo supplementation(8-11 weeks)
- Changes in subjective feeling during and after exercise between L-arginine supplementation and placebo supplementation(8-11 weeks)
- Changes in muscle oxygenation between L-arginine supplementation and placebo supplementation(8-11 weeks)
- Changes of amino acid and nitric oxide biomarkers levels in plasma between L-arginine + L-citrulline supplementation and placebo supplementation(8-11 weeks)
研究者
Stephen Bailey
Lecturer in Sport ad Exercise Nutrition
Loughborough University