Gut Microbiota in Patients With Moyamoya Disease
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 120
- 试验地点
- 1
- 主要终点
- Analysis of differences in intestinal microbiota between MMD patients and the control group
研究概览
简要总结
This study is to find out the association between gut microbiota and moyamoya disease.
详细描述
Moyamoya disease (MMD) is a rare cerebrovascular disorder, it is a major cause of stroke in children and young adults in Japan, Korea, and China, but its pathogenesis is not yet clear. Dysbiosis of gut microbiota may play a role in the pathological change of MMD. In this research, the investigators would perform metagenomic sequencing to find the characteristics of gut microbiota of MMD and to explore the correlations with metabolic, immune, and clinical symptoms. This research will not only help the understanding of the pathophysiology of MMD, but also may provide a reference for the selection of clinical treatment options.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 2 Years 至 60 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Diagnosis of MMD by DSA according to the Japanese guidelines published in 2012,
- •Stenosis or occlusion of the terminal internal carotid and the proximal middle and anterior cerebral arteries and unilateral or bilateral involvement
- •Age: 2-60 years old.
排除标准
- •Moyamoya syndrome,
- •Evidence of gastrointestinal infection/ inflammation/ obstruction
- •History of partial or total resection of small or large bowel, as well as gut reanastomosis,
- •Use of antibiotics within 2 weeks prior to symptoms onset,
- •Gastrointestinal malignancy
- •Any hospitalization within 3 months before recruitment
结局指标
主要结局
Analysis of differences in intestinal microbiota between MMD patients and the control group
时间窗: Through study completion，eight months.
The Spearman correlation coefficient between genes will be calculated, and genes with strong correlation will be grouped into one cluster, as a CAG. The abundance of CAGs in each sample will be determined Furthermore, the significantly enriched species in the control and MMD groups will be enumerated for network display.
Analysis of functional differences in the intestinal microbiota of MMD patients in comparison to the control group
时间窗: Through study completion，eight months.
The LEfSe discriminant analysis will be used to screen for significant differences between groups. The dimensionality reduction will be implemented by LDA, and the impact of function difference will be evaluated to obtain the LDA score and identify significantly different functions between groups.
Analysis of metabolites of the intestinal microbiota of MMD patients in comparison to the control group
时间窗: Through study completion，eight months.
Metabolites of the intestinal microbiota between two groups
Diversity analysis of genes and species between MMD patients and the control group
时间窗: Through study completion，eight months.
Based on the gene and species composition of each sample, the Chao1 and Shannon indexes, as well as the observed OTUs (operational taxonomic units), will be calculated in order to identify the differences in gene and species diversity for each group.
次要结局
- Correlation analysis between gut microbiota in MMD patients and neuroimaging markers and cognitive status.(Through study completion，eight months.)
- Correlation analysis between gut microbiota in MMD patients and inflammatory cytokines.(Through study completion，eight months.)
- The differences of composition of gut microbiota among the genotypes (RNF213 p.R4810K)(Through study completion，eight months.)