The Role of Acute Total Sleep Deprivation in the Regulation of Metabolism, Neuroendocrine Responses, and Behavioral Measures
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 17
- Locations
- 2
- Primary Endpoint
- Gene expression and DNA methylation
Study Overview
Brief Summary
The study proposes to investigate whether acute total deprivation affects metabolism as measured through blood and peripheral tissues. Its aim is also to investigate how acute total sleep deprivation affects neurodegenerative markers, as well as hormones, memory performance and aspects of appetite regulation.
Detailed Description
It is predicted that acute total sleep deprivation will affect gene expression and DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and affect other neuroendocrine markers and hormones in a negative manner. It is further predicted that sleep deprivation will decrease participants' memory performance.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 28 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Age 18-28y
- •Healthy (self-reported) and not on medication
- •Non-smoking
- •Normal sleep-wake rhythm (i.e. 7-8 h per night, self-reported via diaries)
Exclusion Criteria
- •Major illness
- •Taking any serious medications
- •Any sleep conditions (e.g. irregular bedtimes, sleep complaints)
- •Any dietary issues with the food items provided
- •Current or history of endocrine, neurological or psychiatric disorders
- •Shift work in the preceding three months or for a long duration
- •Time travel over a significant number of time zones in the preceding two months
- •Too much weight gain or weight loss in the preceding three months
Outcomes
Primary Outcomes
Gene expression and DNA methylation
Time Frame: Change from baseline (ie. around 1930 in the evening - before sleep intervention) to 12 hours later (around 0730 in the morning after the nighttime intervention), and to 15 hours later (around 1030 in the morning after the nighttime intervention)
This study has been designed to measure the changes in gene expression and DNA methylation in circulating blood, i.e. mainly of white blood cells with active transcription and DNA regulation, and how this relates to possible changes in peripheral tissues involved in metabolism.
Secondary Outcomes
- Appetitive evaluation(Change in appetitive ratings after the sleep intervetion (from around 0700 in the morning after the nighttime intervention), repeated each hour)
- Circulating hormone and neuromolecular levels(Change in circulating hormone levels from baseline (ie. around 1930 in the evening - before sleep intervention) to 12 hours later (around 0730 in the morning after the nighttime intervention))
- Inhibitory task(Change in cognitive inhibitory performance at around 0810 hours in the morning following the respective nighttime intervention.)
- Portion Size Task(Change in selected portion size in the morning, at around 0830 hours in the morning following each nighttime intervention, and 2h30min later, i.e. 30 min after consuming an Oral glucose tolerance test, i.e. around 1100 hours)