Glucose Metabolism in Subjects With Aldosterone-Producing Adenomas
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Vanderbilt University
- Enrollment
- 10
- Locations
- 1
- Primary Endpoint
- Change in Acute Glucose-stimulated Insulin Secretion
Study Overview
Brief Summary
This observational study tests the hypothesis that endogenous aldosterone impairs insulin secretion and insulin sensitivity in subjects with primary aldosteronism.
Detailed Description
The week of each study period, subjects will be provided a standard 160mmol/d sodium diet for 6-8 days to control for inter-individual sodium intake.
In period 1, subjects will report after 5 days of controlled sodium diet for a hyperglycemic clamp study (to measure insulin secretion). Subjects will continue the study diet, and then return for a hyperinsulinemic-euglycemic clamp study (to measure insulin sensitivity).
After completion of period 1 assessment, subjects will undergo adrenalectomy by our endocrine surgeons or initiate medical treatment, according to routine clinical care.
In period 2, the investigators will repeat the studies in the same manner as period 1, 3 to 12 months after adrenalectomy or initiation of medical treatment.
Study Design
- Study Type
- Observational
Eligibility Criteria
- Ages
- 18 Years to 70 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Ambulatory subjects, 18 to 70 years of age, inclusive
- •For female subjects, the following conditions must be met:
- •postmenopausal status for at least 1 year, or
- •status-post surgical sterilization, or
- •if of childbearing potential, utilization of adequate birth control and willingness to undergo urine beta-hcg testing on every study day.
- •Primary aldosteronism determined by both:
- •Biochemical hyperaldosteronism defined as either:
- •Plasma aldosterone ≥15 ng/dL
- •or aldosterone-to-renin ratio of ≥30 if on ACE inhibitor
- •or aldosterone-to-renin ratio of ≥40 in absence of an ACE inhibitor
- •Positive suppression test defined as either:
- •failure to suppress aldosterone to <7ng/dL after intravenous 0.9% saline infusion over 2 hours
- •failure to suppress 24-hour urinary aldosterone excretion to <12 µcg with simultaneously documented urine sodium excretion >200 mmol.
Exclusion Criteria
- •Subjects presenting with any of the following will not be included in the study:
- •Previously diagnosed type 1 Diabetes
- •Type II Diabetes, as defined by ADA criteria:
- •Hemoglobin A1C ≥6.5%
- •Fasting plasma glucose ≥126mg/dl (7.0mmol/l)
- •2-hour 75g oral glucose tolerance test (OGTT) plasma glucose ≥200mg/dl (11.1 mmol/l) d. Current treatment with anti-diabetic medication(s)
- •Impaired renal function [estimated glomerular filtration rate (eGFR) of <30ml/min] as determined by the four-variable Modification of Diet in Renal Disease (MDRD) equation, where serum creatinine (Scr) is expressed in mg/dl and age in years.
- •Prior allergies to medications used in the study protocol (e.g. L-arginine, potassium chloride, insulin), or to drugs within the same class.
- •Screening plasma potassium >5.5 mmol/L or sodium <135 mmol/L
- •Cardiovascular disease such as recent (<6 months) myocardial infarction, presence of angina pectoris, significant arrhythmia, congestive heart failure (LV hypertrophy acceptable), deep vein thrombosis, pulmonary embolism, second or third degree heart block, mitral valve stenosis, aortic stenosis or hypertrophic cardiomyopathy
- •Breast-feeding
- •Treatment with anticoagulants
- •History of serious neurologic disease such as cerebral hemorrhage, stroke, seizure, or transient ischemic attack
- •History or presence of immunological or hematological disorders
- •Diagnosis of asthma requiring use of inhaled beta agonist >1 time per week
- •Clinically significant gastrointestinal impairment that could interfere with drug absorption
- •Impaired hepatic function [aspartate amino transaminase (AST) and/or alanine amino transaminase (ALT) >2.0 x upper limit of normal range]
- •Hematocrit <35%
- •Any underlying or acute disease requiring regular medication which could possibly pose a threat to the subject or make implementation of the protocol or interpretation of the study results difficult, such as arthritis treated with non-steroidal antiinflammatory drugs
- •Treatment with chronic systemic glucocorticoid therapy (more than 7 consecutive days in 1 month)
- •Treatment with lithium salts
- •History of alcohol or drug abuse
- •Treatment with any investigational drug in the 1 month preceding the study
- •Mental conditions rendering the subject unable to understand the nature, scope and possible consequences of the study
- •Inability to comply with the protocol, e.g., uncooperative attitude, inability to return for follow-up visits, and unlikelihood of completing the study
Arms & Interventions
Adrenalectomy
Subjects will undergo assessment before and after adrenalectomy for treatment of primary aldosteronism
Intervention: Adrenalectomy (Other)
Medical Therapy
Subjects will undergo assessment before and after medical treatment of primary aldosteronism
Intervention: mineralocorticoid receptor antagonist (Drug)
Outcomes
Primary Outcomes
Change in Acute Glucose-stimulated Insulin Secretion
Time Frame: Change from Baseline vs. 3-12 months after intervention
measured by hyperglycemic clamp
Change in Insulin Sensitivity Index
Time Frame: Change from Baseline vs. 3-12 months after intervention
measured by hyperinsulinemic-euglycemic clamp
Change in Disposition Index (product of Insulin sensitivity index and acute insulin secretion)
Time Frame: Change from Baseline vs. 3-12 months after intervention
Product of insulin sensitivity and insulin secretion
Secondary Outcomes
- Suppression of Hepatic glucose production(Change from Baseline vs. 3-12 months after intervention)
Investigators
James Matt Luther
James M Luther MD, MSCI
Vanderbilt University