Local and Systemic Cytokine Profiles in Children With Obstructive Sleep Apnea and Controls
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 2
- 主要终点
- levels of cytokines released from PBMCs of OAS subjects and controls
研究概览
简要总结
The purpose of this study is to compare the cytokine profile of tonsillar and peripheral blood mononuclear cells in patients with obstructive sleep apnea (OSA) and to compare the blood mononuclear cell cytokine secretion profile between patients with obstructive sleep apnea and controls without apnea.
详细描述
We will recruit subjects with OSA about to undergo adenotonsillectomy and collect a blood sample and portion of their tonsils after the induction of general anesthesia. The mononuclear cells will be isolated from both these tissues and stimulated with different markers and cytokines assayed and compared. We will also recruit patients with no symptoms of OSA undergoing unrelated elective surgery and ascertain the absence of OSA by having the parents fill out a sleep questionnaire. We will then collect blood samples from these patients after the induction of anesthesia and examine the cytokine secretion profile and compare it to that of the children with OSA.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Months 至 12 Years（Child）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •OSA documented by polysomnography and clinical symptoms.
- •Patients with no symptoms of OSA and negative sleep questionnaire.
排除标准
- •Significant systemic disease except mild asthma
结局指标
主要结局
levels of cytokines released from PBMCs of OAS subjects and controls
时间窗: just prior to surgery
次要结局
- cytokine level comparison between PBMCs and tonsillar lymphocytes within OAS groups(at time of surgery)