MedPath

Metabolomic Profile in Breastfed Late Preterm Infants

Completed
Conditions
Breast Milk Collection
Postbiotic Supplemented Formula Milk
Formula Milk
Interventions
Other: Milk
Registration Number
NCT06052592
Lead Sponsor
Ospedale Buon Consiglio Fatebenefratelli
Brief Summary

Exclusive breast milk is recommended from birth to 6 months of life to promote the development of a balanced intestinal microbiota. Human milk provides several bioactive components, from natural probiotics such as Bifidobacterium spp. and Lactobacillus spp., to their metabolites which colonize the intestine of the newborn. However, if breast milk is not available or insufficient, it is used formula milk as a substitute. Infant formula can be supplemented with postbiotics to promote maturation of immune, metabolic and microbial components, similar to breast milk. Postbiotics are preparations composed of both microbial constituents and their metabolites, produced during fermentation.

Detailed Description

A balanced nutrition starting from early childhood significantly influences growth and psychomotor development. Exclusive breast milk is the recommended nutritional choice from birth to 6 months of life, as it guarantees everything necessary for growth, maturation, protection from infections, promoting the development of a balanced intestinal microbiota. Several prenatal and perinatal factors including the type of delivery, the use of antibiotics, diet and other environmental factors, can influence the microbial colonization of the newborn. Thus, it is generally accepted that the gut microbiota of the healthy, full-term, vaginally born, breastfed infant is the gold standard for a favorable microbial composition in the early years of life. Human milk provides several bioactive components, from natural probiotics such as Bifidobacterium spp. and Lactobacillus spp., to their metabolites ("natural postbiotics"), which colonize the intestine of the newborn. These metabolites regulate the development of the immune system and attenuate the inflammatory processes. However, if breast milk is not available or insufficient to meet the nutritional needs of the infant, formula milk is proposed and used as a substitute. Given the benefits, it is essential that infant formula is as close to human milk as possible, providing bioactive substances that target gut and immune system health. Current research focuses on optimizing artificial formulas, with the aim of resembling human milk in composition and functionality, with some on the market already including probiotics, prebiotics, synbiotics and postbiotics. However, it still remains to be clarified which is the best formulation and the exact consequences on the immune, metabolic and microbial system of the newborn. In particular, postbiotics are preparations composed of both microbial constituents and their metabolites, produced during fermentation. It has already been highlighted in the literature that the enrichment of formula milk with post-biotics would seem to offer advantages for feeding term infants. Furthermore, it promotes an immune, metabolic and microbial component maturation, similarly to human milk, thus making postbiotic supplements very promising and interesting in the nutrition of newborns and infants.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
54
Inclusion Criteria
  • Weight appropriate to gestational age (percentiles >10th and <90th)
  • Written informed consent
Exclusion Criteria
  • Cardiological diseases
  • Liver disease
  • Gastrointestinal diseases with malabsorption
  • Endocrinological diseases
  • Perinatal infections
  • Metabolic and genetic diseases
  • Born to mothers with endocrinological and metabolic diseases
  • Withdrawal of informed consent

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Group CMilkNewborns fed with exclusive breast milk
Group AMilkNewborns fed with formula milk supplemented with 20 mg/day of fermented FOS from Lactobacillus paracasei strain CNCM I-5220 and Vitamin D (0.5 ml of SMART D3 MATRIX)
Group BMilkNewborns fed with formula milk
Primary Outcome Measures
NameTimeMethod
Evaluation of metabolomic profile by liquid chromatography mass spectrometry in breastfed late preterm infants3 months after delivery

To detect the differences in the metabolome of the newborns

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (2)

Department of Pharmacy, University of Salerno

🇮🇹

Salerno, Naples, Italy

Department of Woman and Child, Buon Consiglio Fatebenefratelli Hospital

🇮🇹

Napoli, Italy

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