Retrospective study on peripheral blood natural killer(pNK) cells as surrogate measure for uterine natural killer(uNK) cells
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- pNK and uNK cell
研究概览
简要总结
Summary :
Natural killer cells are one of the major components of the innate immune system. The natural killer cells are present in peripheral blood as well as in some target organs like spleen, liver, uterus etc. Uterine natural killer cells secrete many cytokines and growth factors which may play a role in implantation. Alteration in the number of uterine natural killer cells subset has been suggested as a cause for recurrent miscarriage as well as implantation failure. Measurement of uterine natural killer cells is technologically challenging and requires invasive endometrial biopsy.
The purpose of this study was to find out whether there is any correlation between the number peripheral blood NK cells and the number of uterine NK cells, so that the results of measuring the peripheral blood natural killer cells can be extrapolated to the uterine natural killer cells thereby avoiding the invasive testing.
We will be doing a retrospective analysis of the hospital records between May 2017 to October 2017. Infertile patients between ages 25 to 38years who have undergone measurement of peripheral blood and uterine natural killer cells will be included. Women with previous history of abortion or recurrent implantation failure will be included in this study. Women with irregular cycles, women with antiphospholipid antibody syndrome and who were on oral contraceptive pills will be excluded. The patients included had undergone both the peripheral blood and uterine (endometrial) sample on the same day in the mid luteal phase of menstrual cycle(Day 22 to Day 26 of menstrual cycle). The percentage of CD 56 bright and CD 16 dim as well as CD 56 dim and CD 16 bright in the samples were analyzed by flow cytometry.
By this study we will know whether there is a correlation between the number of peripheral and uterine NK cells in the secretory phase and if there is a correlation we can avoid the invasive uterine biopsy.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 28.00 Year(s) 至 35.00 Year(s)（—）
- 性别
- Female
入选标准
- •1.Infertile women of ages 28 to 35 years who had
- •History of two or more abortions
- •History of recurrent implantation failure (failure to achieve a clinical pregnancy after transfer of at least four good-quality embryos in a minimum of three fresh or frozen cycles in a woman).
排除标准
- •Women with irregular cycle
- •Women on Oral contraceptive pills
- •Women with Antiphospholipid antibody syndrome (APLA) positive.
结局指标
主要结局
pNK and uNK cell
时间窗: Cross sectional study, only once sample will be collected from infertile women
次要结局
未报告次要终点