Immunohistochemical Evaluation of Lymphocyte Subpopulations in Peripheral Giant Cell Granuloma and Healthy Gingival Tissues: A Within-Subject Controlled Study
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 28
- 试验地点
- 1
- 主要终点
- Proportion of Lymphocyte Subpopulations
研究概览
简要总结
Peripheral giant cell granuloma (PGCG) is a non-neoplastic inflammatory lesion of gingival tissues. This study aims to evaluate and compare lymphocyte subpopulations in PGCG tissues and adjacent healthy gingival tissues obtained from the same individuals.
A total of 28 systemically healthy, non-smoking patients diagnosed with PGCG are included in this study. Biopsy samples are collected during surgical excision of PGCG lesions, and corresponding healthy gingival tissues are obtained from the same patients.
Immunohistochemical analysis is performed to assess lymphocyte subpopulations, including CD3, CD4, CD8, CD20, and CD79a. The proportions of these cells are compared between diseased and healthy tissues.
详细描述
Peripheral giant cell granuloma (PGCG) is a relatively common non-neoplastic inflammatory lesion of the gingiva characterized by multinucleated giant cells within a vascularized stroma. Lymphocyte subpopulations, including T- and B-cells, play a significant role in the immunopathogenesis of gingival and periodontal diseases.
The evaluation of lymphocyte subpopulations in gingival tissues is an established method for understanding local immune responses. However, limited data are available regarding lymphocyte profiles in PGCG lesions. Therefore, this study aims to investigate and compare lymphocyte subpopulations in PGCG and healthy gingival tissues.
This study is designed as a within-subject controlled observational study including 28 systemically healthy and non-smoking patients diagnosed with PGCG. Biopsy samples are obtained from PGCG lesions during surgical excision, and corresponding healthy gingival tissues are collected from the same individuals.
Tissue specimens are processed using standard histological procedures and analyzed by immunohistochemical methods. The presence and proportion of CD3, CD4, CD8, CD20, and CD79a positive cells are evaluated. The proportion of positively stained cells is calculated as the percentage of positive cells relative to the total number of counted cells.
The results are compared between PGCG tissues and healthy gingival tissues to identify differences in lymphocyte subpopulations and to better understand the immunopathological characteristics of PGCG.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Systemically healthy individuals
- •Non-smokers
- •Patients diagnosed with peripheral giant cell granuloma
- •Individuals requiring surgical excision of PGCG
- •Patients providing written informed consent
排除标准
- •Presence of systemic diseases
- •Smoking habit
- •Use of medications associated with gingival enlargement
- •History of periodontal treatment within the last 6 months
- •Pregnant or lactating individuals
研究组 & 干预措施
PGCG Tissue
Biopsy samples obtained from peripheral giant cell granuloma lesions during surgical excision.
干预措施: Immunohistochemical Analysis (Diagnostic Test)
Healthy Gingival Tissue
Biopsy samples obtained from adjacent healthy gingival tissues of the same patients.
干预措施: Immunohistochemical Analysis (Diagnostic Test)
结局指标
主要结局
Proportion of Lymphocyte Subpopulations
时间窗: At the time of tissue analysis (baseline)
The proportion of CD3, CD4, CD8, CD20, and CD79a positive cells is determined by calculating the percentage of positively stained cells relative to the total number of counted cells in gingival tissue samples.
次要结局
- CD4/CD8 Ratio(At the time of tissue analysis (baseline))
研究者
Alparslan Dilsiz
Principal Investigator
Ataturk University