Investigation of Supragingival Plaque Microbiome Using Next Generation Sequencing in Early Childhood Caries
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- V4-16S rRNA and ITS1 rRNA sequencing
研究概览
简要总结
The purpose of this study was to characterize the supragingival plaque microbiome in children with early childhood caries and identify potential microorganisms associated with caries.
详细描述
Sixty children with and without early childhood caries were recruited from Trakya University Faculty of Dentistry Department of Pediatric Dentistry. Oral examinations were performed by the same examiner according to diagnostic criteria of World Health Organisation (WHO) and The International Caries Detection and Assessment System (ICDAS) . Mothers' completed a structured questionnaire on the socio-demographic informations, child's feeding practices and oral hygiene habits. After the oral examination, supragingival plaque samples were collected from the posterior tooth surfaces. Supragingival plaque samples were examined with the Illumina MiSeq new generation sequencing method.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Other
入排标准
- 年龄范围
- 36 Months 至 71 Months（Child）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •36-71 months old children
- •No history of any medical problem
- •No history of antibiotic in the three months prior to sampling
- •Any sign of early childhood caries,
- •Caries free children
- •Mothers accepted to complete a structured questionnaire
- •Children who cooperate for dental examination and sample collection
排除标准
- •Children older than 71 months
- •Medically compromised
- •Children with antibiotic use history at last three months
- •Children refused dental examination and plaque collection
- •Mothers' refused to complete questionnare
结局指标
主要结局
V4-16S rRNA and ITS1 rRNA sequencing
时间窗: 6 months
Supragingival plaque samples were collected from the tooth surfaces using a sterile periodontal curette. DNA extraction was performed at Sugenomik Biotechnology Laboratory for library preparation and paired-end Illumina MiSeq PE250 sequencing of the V4 region of bacterial 16S rRNA and fungal ITS1 (internal transcribed spacer 1) rRNA genes.
次要结局
未报告次要终点
研究者
ŞİRİN GÜNER ONUR
Assist. Prof. Dr.
Trakya University