Investigation of Supragingival Plaque Microbiome Using Next Generation Sequencing in Early Childhood Caries
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Early Childhood Caries
- Sponsor
- Trakya University
- Enrollment
- 60
- Locations
- 1
- Primary Endpoint
- V4-16S rRNA and ITS1 rRNA sequencing
- Status
- Completed
- Last Updated
- 5 years ago
Overview
Brief Summary
The purpose of this study was to characterize the supragingival plaque microbiome in children with early childhood caries and identify potential microorganisms associated with caries.
Detailed Description
Sixty children with and without early childhood caries were recruited from Trakya University Faculty of Dentistry Department of Pediatric Dentistry. Oral examinations were performed by the same examiner according to diagnostic criteria of World Health Organisation (WHO) and The International Caries Detection and Assessment System (ICDAS) . Mothers' completed a structured questionnaire on the socio-demographic informations, child's feeding practices and oral hygiene habits. After the oral examination, supragingival plaque samples were collected from the posterior tooth surfaces. Supragingival plaque samples were examined with the Illumina MiSeq new generation sequencing method.
Investigators
ŞİRİN GÜNER ONUR
Assist. Prof. Dr.
Trakya University
Eligibility Criteria
Inclusion Criteria
- •36-71 months old children
- •No history of any medical problem
- •No history of antibiotic in the three months prior to sampling
- •Any sign of early childhood caries,
- •Caries free children
- •Mothers accepted to complete a structured questionnaire
- •Children who cooperate for dental examination and sample collection
Exclusion Criteria
- •Children older than 71 months
- •Medically compromised
- •Children with antibiotic use history at last three months
- •Children refused dental examination and plaque collection
- •Mothers' refused to complete questionnare
Outcomes
Primary Outcomes
V4-16S rRNA and ITS1 rRNA sequencing
Time Frame: 6 months
Supragingival plaque samples were collected from the tooth surfaces using a sterile periodontal curette. DNA extraction was performed at Sugenomik Biotechnology Laboratory for library preparation and paired-end Illumina MiSeq PE250 sequencing of the V4 region of bacterial 16S rRNA and fungal ITS1 (internal transcribed spacer 1) rRNA genes.