Non-stick Patch of Mesothelial Cells in a Hydrogel Matrix : Exploratory Analysis Assessing the Morphotype and Mesothelial Phenotype After Culture From 2 Cells Sources of Human Origin.
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 入组人数
- 6
- 试验地点
- 1
- 主要终点
- Morphotype/Phenotype expression
研究概览
简要总结
The objective of this study, which will be realised in vitro on human samples, is to test 2 cell sources isolated from peritoneum for adult mesothelial cells (peritoneal samples) and from adipose tissue for the adipose stem cells (adipose tissue samples). The latter will be differentiated towards the mesothelial phenotype and morphotype. The 2 routes will be compared and the most satisfactory one will be chosen to make a non-stick patch.
详细描述
- Patient information and collection of a signed informed consent
- Clinical data collection: surgery date, age, weight, height, medical and surgical history
- Prospective collection of peritoneal and adipose biopsies of patients during their surgery at the Oscar Lambret Center (Dr L. BRESSON)
- Transportation of the samples to the 1008 INSERM Unit.
- Isolation of cells from the samples : mesothelial cells from the peritoneum and adipose stem cells from adipose tissue
- Cell culture and expansion of mesothelial cells; and cell culture and differentiation of adipose stem cells
- Identification by the morphotype and phenotype of mesothelial cells
- Comparison of the results according to the cell source, peritoneum or adipocyte, to the purity of cells and to the efficacy of the expansion
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Aged more than 18 years old,
- •Planned surgery For peritoneal samples : abdominal surgery (laparotomy or coelioscopy) For adipose tissue samples : breast reconstructive surgery (lipomodelage)
- •Informed and signed consent
排除标准
- •Pregnant or breastfeeding woman
- •Patient under guardianship or tutorship
- •For peritoneal samples : previous abdomino-pelvic radiotherapy or abdominal surgery ; peritoneal carcinosis
研究组 & 干预措施
Biological samples collection
-Peritoneal samples : will be collected during abdominal surgery
A biopsy from 1 to 2 cm2 will be performed in healthy peritoneum
-Adipose tissue samples : will be collected during surgery for breast reconstruction
干预措施: Biological samples collection (Procedure)
结局指标
主要结局
Morphotype/Phenotype expression
时间窗: 1 year
Mesothelial morphotype and phenotype expression of the 2 types of cells after culture
次要结局
未报告次要终点