Understanding the role of the pigment producing cells in the formation of keloids
- Conditions
- Other hypertrophic disorders of the skin,
- Registration Number
- CTRI/2021/12/038951
- Lead Sponsor
- IADVL Indian Association of Dermatology Venereology and Leprosy
- Brief Summary
Keloids- benign fibroproliferative tumours that either arise spontaneously or afterinjury - are characterized by hyperproliferative fibroblasts with an increasedtendency to differentiate into myofibroblasts. This deregulation of fibroblastfunction causes excessive deposition of collagen in the dermis leading to a visibleraised scar which may spread beyond the borders of the initial injury. Theprevalence of keloids is significantly higher in individuals with skin ofcolour; around 16 % of people in darkly pigmented populations like AfricanAmericans and Asian Americans are known to be affected with keloids. On theother hand, keloids rarely develop in Caucasians, albinos and individuals with vitiligo(on the depigmented patch), all of whom have a lower pigmentation content.
Sincekeratinocytes, melanocytes and fibroblasts maintain skin homeostasis by closelyinteracting with each other via several autocrine and paracrine signallingpathways, w***e hypothesised that either melanocytes per se and/or themelanin content therein could influence fibroblast biology, leading to thedevelopment or spread of keloids.***
To testthe role of perturbed melanocytes - fibroblasts cross talk in keloidpathogenesis, we propose to carry out the following **objectives**:
A) Understanding Fibroblast-Melanocyte cross-talk inkeloid pathogenesis:
A.1) Understanding the effect of melanocyte-conditioned media(melanocyte secretome) on fibroblast biology (trans-well co-culture set up)
A.2) Understanding the effect of modulation of melanin content inmelanocytes on the fibroblast biology
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Not Yet Recruiting
- Sex
- All
- Target Recruitment
- 20
Individuals diagnosed with keloid (scar spread beyond original borders and no spontaneous regression within 1 year) Having taken no treatment for keloids before (Treatment naïve) Not on any immunomodulator drugs for other conditions Male and female Above 18 years age .
Mixed or unclear diagnosis Pregnant/lactating females Patients who refuse to participate in the study Patients having other diseases like diabetes, or with communicable diseases.
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method The proposed study is an exploratory study to understand the relation between melanocytes and fibroblasts in keloids. It is not a clinical trial, and hence no success/failure is involved. 1 year Understanding the effect of modulation of melanin content in melanocytes on the fibroblast biology 1 year
- Secondary Outcome Measures
Name Time Method Understanding the effect of melanocyte-conditioned media (melanocyte secretome) on fibroblast biology (trans-well co-culture set up). 1 year
Trial Locations
- Locations (1)
Dr. D.Y. Patil Medical College, Hospital and research centre
🇮🇳Pune, MAHARASHTRA, India
Dr. D.Y. Patil Medical College, Hospital and research centre🇮🇳Pune, MAHARASHTRA, IndiaDr Aayush GuptaPrincipal investigator9545711211aayushggupta@gmail.com