The Influence of Sampling Site When Assessing Glucose Tolerance or Insulin Sensitivity With Oral Glucose Ingestion

Not Applicable

Completed

• Conditions: Hyperglycemia; Insulin Sensitivity
• Interventions: Behavioral: Rest; Behavioral: Exercise; Other: Dorsal Hand Vein Cannulation (heated hand technique); Other: Venous Cannulation

• Registration Number: NCT02852044
• Lead Sponsor: University of Bath

Brief Summary

For decades, it has been known that post-meal blood glucose concentrations were associated with the risk of T2D, which was reflected in early diagnostic guidelines. The oral glucose tolerance test (OGTT) has been used since at least 1923 and has remained the most common test for assessing glucose tolerance. Arterial blood (or arterialised blood using heated hand technique) is most appropriate for determining glucose tolerance and insulin sensitivity since this best represents the concentrations of metabolites and hormones that peripheral tissues are exposed to. It is essential to investigate whether venous blood (sometimes used during an OGTT) is representative of arterialised blood during an OGTT, and under different metabolic conditions.

The investigators want to understand whether OGTT-derived insulin sensitivity indices differ from venous and arterialised blood; and 2) investigate whether metabolic status (i.e. rest vs lower-limb exercise) influences the difference between forearm venous and arterialised concentrations of glucose and insulin during an OGTT.

Detailed Description

Not available

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study Start: 2015-11
• Primary Completion: 2016-04
• Study First Submitted: 2016-07-28
• Study First Submitted QC: 2016-08-01
• Study First Posted: 2016-08-02
• Study Completion: 2016-09
• Status Verified: 2016-10
• Last Update Submitted: 2016-10-24
• Last Update Posted: 2016-10-25

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

• Aged 18-49 years
• Able and willing to safely comply with all study procedures
• Able to provide written informed consent for participation

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Exclusion Criteria

• Diagnosis of any bleeding disorder or taking medication which impacts blood coagulation
• The presence of any contraindications to maximal exercise testing, as determined using a physical activity readiness questionnaire (PAR-Q).
• Any diagnosis of metabolic disease (i.e. cardiovascular disease or type 2 diabetes).

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Rest	Dorsal Hand Vein Cannulation (heated hand technique)	Remain rested prior to the oral glucose tolerance test
Rest	Rest	Remain rested prior to the oral glucose tolerance test
Rest	Venous Cannulation	Remain rested prior to the oral glucose tolerance test
Exercise	Dorsal Hand Vein Cannulation (heated hand technique)	Complete exercise prior to the oral glucose tolerance test
Exercise	Venous Cannulation	Complete exercise prior to the oral glucose tolerance test
Exercise	Exercise	Complete exercise prior to the oral glucose tolerance test

Primary Outcome Measures

Name	Time	Method
Postprandial plasma glucose concentrations (area under the concentration-time curve)	120 mins
OGTT-derived insulin sensitivity	120 mins

Secondary Outcome Measures

Name	Time	Method
Fasting plasma glucose concentrations	5 mins
Substrate utilization for energy provision	180 mins
Fasting plasma insulin concentrations	5 mins
Fasting plasma lactate concentrations	5 mins
Fasting plasma triglyceride concentrations	5 mins
Postprandial plasma insulin concentrations (area under the concentration-time curve)	120 mins
Postprandial plasma triglyceride concentrations (area under the concentration-time curve)	120 mins
Postprandial plasma lactate concentrations (area under the concentration-time curve)	120 mins

Trial Locations

Locations (1)

Department for Health, University of Bath; 🇬🇧 Bath, United Kingdom