Mechanisms of Glucose Lowering Effect of Colesevelam HCl

Not Applicable

Completed

Conditions: Diabetes

Interventions: Drug: Placebo
Drug: Colesevelam HCL

Registration Number: NCT00596427

Lead Sponsor: Carine Beysen

Brief Summary: The mechanism by which colesevelam HCl lowers glucose is not known. Knowledge of the potential mechanism of action is important for defining the role of the drug among oral antidiabetic agents available for use in subjects with diabetes. The objective of this study is to provide insight into the mechanisms of action of colesevelam HCl in T2DM. The mechanisms of interest include hepatic insulin sensitivity, rate of appearance of exogenous glucose and changes in incretin hormone concentrations.

Detailed Description: Colesevelam HCl (marketed in the U.S. as WelChol®) is a non-absorbed polymer that binds bile acids in the intestine, impeding their reabsorption, and is indicated to lower low-density lipoprotein cholesterol (LDL-C) in subjects with hypercholesterolemia. As the bile acid pool becomes depleted, the hepatic enzyme cholesterol 7-(alpha)-hydroxylase is upregulated, increasing the conversion of cholesterol to bile acids. This causes an increased demand for cholesterol in the liver, resulting in the dual effect of increasing transcription and activity of the cholesterol biosynthetic enzyme, hydroxymethyl-glutaryl-coenzyme A (HMG CoA) reductase, and increasing the number of hepatic low-density lipoprotein (LDL) receptors. These compensatory effects increase the clearance of LDL-C from the blood, decreasing serum LDL C levels (1; 2).

Recently, it has been shown that colesevelam HCl also improves glycemic control in subjects with T2DM who are not controlled adequately on metformin, sulfonylurea or a combination of the two drugs (3). The mechanism of action for glucose lowering is not known. Improved glycemic control with colesevelam HCl treatment could be due to any of several mechanisms. Colesevelam HCl could reduce hepatic insulin resistance and lead to a decrease in hepatic glucose production (HGP). The observation by Schwartz et al (4) of significantly reduced fasting plasma glucose concentrations in colesevelam-treated T2DM patients suggests such a reduction in HGP, as fasting hyperglycemia is a direct function of HGP. Colesevelam HCl could also decrease post-prandial glucose absorption. Changes in glucose absorption with other bile acid sequestrants (BAS) (5) and bile acids (6) have been reported.

With regard to molecular mediators of the colesevelam effect on glucose metabolism, there is considerable evidence emerging about the role of bile acids and nuclear transcription factors, such as the farnesyl X receptor (FXR), in the regulation of glucose and lipid metabolism (7) (8) (9-15). Changes in cellular lipids or nuclear hormone receptors might directly alter HGP although mechanisms leading to changes in hepatic lipid and glucose metabolism by colesevelam HCl have not previously been investigated.

Significant changes in cholesterol and bile acid synthesis rates are expected with colesevelam treatment. BAS treatment can alter the transhepatic flux and compositional profile of the circulating bile acid pool (16), and thus its hydrophobicity, and this may effect the activation of nuclear receptors, including FXR (17; 18). Determination of the effect of colesevelam treatment on bile acid synthesis may provide evidence for its metabolic effects. The effects on hepatic fatty acid synthesis (de novo lipogenesis or DNL) have not been investigated and may provide further evidence for a metabolic effect of colesevelam.

Specific hypotheses about its mode of action will be tested, focusing on hepatic glucose metabolism and intestinal glucose absorption.

Recruitment & Eligibility

Status: COMPLETED

Sex: All

Target Recruitment: 60

Inclusion Criteria

Subjects meeting the following criteria at the Screening Visit will be eligible to participate in the trial:

Have given written informed consent
Male or Female
1. Females of childbearing potential who are on approved birth control method:
  
  oral, injectable, or implantable hormonal contraceptives; intrauterine device; diaphragm plus spermicide or female condom plus spermicide
2. Females of non-childbearing potential: hysterectomy, tubal ligation 6 months prior screening or post-menopausal for at least 1 year
Previously diagnosed or newly diagnosed with T2DM
Age 30 to 70 years, inclusive
BMI ≥ 18.5 kg/m2 and ≤ 40 kg/m2
HbA1C 7-10%, inclusive (exceptions between 6.7-7% may be enrolled with prior approval of SPONSOR)
Fasting plasma glucose < 300 mg/dL
Diet controlled or on stable dose of a sulfonylurea and/or meglitinides and/or metformin for ≥ 90 days before screening
No history of liver, biliary or intestinal disease (AST/ALT < 2X upper limit of normal value)
Normal TSH
Agrees to maintain their regular diet and exercise routine
Agrees to refrain from consumption of alcohol 48 hours prior to start of infusions (week 0 and week 12)

Exclusion Criteria

Subjects are excluded from participation in the study if any of the following criteria apply:

Type 1 diabetes mellitus or history of diabetic ketoacidosis
Treatment with lipid lowering medication other than statins
Treatment with statins that have not been stable for 3 months before screening
Treatment with colesevelam HCl, cholestyramine or colestipol for hyperlipidemia within the last 3 months of screening
Treatment with a thiazolidinedione (TZD) at any time
Treatment with acarbose at any time
Treatment with insulin in the past 6 months
Treatment with antibiotics within the last 3 months
Treatment with any medication affecting liver or intestinal function within the last 3 months
Pregnant
Breastfeeding
Has had unstable weight within the last 3 months of screening (± 5 kg)
History of an allergic or toxic reaction to colesevelam HCl
History of dysphagia, swallowing disorders, or intestinal motility disorder
Serum triglycerides ≥ 350 mg/dL at screening visit (exceptions up to 500 mg/dl may be enrolled with prior approval of SPONSOR)
Serum LDL-C <60 mg/dL at screening visit
Any condition or therapy which, in the opinion of the investigator, poses a risk to the subject or makes participation not in the subject's best interest
Use of any investigational drug within 3 months of screening
Chronic treatment with oral corticosteroids at any time or acute treatment within the last 3 months
History of drug or alcohol abuse, is currently a user (including "recreational use") of any illicit drugs, or has a positive urine drug screen at screening
Donated a unit of blood within 30 days before screening

Study & Design

Study Type: INTERVENTIONAL

Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Placebo tablet 3 tablets 2x/day	Placebo	Type-2 diabetes mellitus patients
Colesevelam HCL 625 mg: 3 tablets 2x/day	Colesevelam HCL	Type-2 diabetes mellitus patients

Primary Outcome Measures

Name	Time	Method
Fasting Glycogenolysis	baseline and 12 weeks	Change from baseline of fasting glycogenolysis after 12 weeks of placebo or colesevelam treatment.
Fasting Gluconeogenesis	baseline and 12 weeks	Change from baseline of fasting gluconeogenesis after 12 weeks of placebo or colesevelam treatment.
Fasting Endogenous Glucose Production (EGP)	baseline and 12 weeks	Changes from baseline of fasting EGP after 12 weeks of placebo or colesevelam treatment.
Rate of Appearance of Exogenous Glucose (Glucose Absorption)	baseline and 12 weeks	Change from baseline of the rate of appearance of oral glucose after 12 weeks of placebo or colesevelam treatment. Mean of values obtained between 0 and 300 min is reported.

Secondary Outcome Measures

Name	Time	Method
Total Glucagon-like Peptide (GLP-1) Area Under the Curve (AUC)	baseline and 12 weeks	Changes from baseline of total GLP-1 AUC after 12 weeks of placebo or colesevelam treatment. AUC values were calculated by the trapezoid method using all results between 0 and 300 minutes
Total Glucose-dependent Insulinotropic Polypeptide (GIP) AUC	baseline and 12 weeks	Changes from baseline of total GIP-1 AUC after 12 weeks of placebo or colesevelam treatment. AUC values were calculated by the trapezoid method using all results between 0 and 300 minutes
Fasting Fractional De Novo Lipogenesis (DNL)	baseline and 12 weeks	Changes from baseline in fasting fractional DNL after 12 weeks of colesevelam or placebo treatment were calculated. Fractional DNL represents the fraction of palmitate in very-low density lipoproteins-triglycerides (VLDL-TG) that was newly synthesized.
Fasting Fractional Cholesterol Synthesis	baseline and 12 weeks	Changes from baseline in fasting fractional cholesterol synthesis after 12 weeks of colesevelam or placebo treatment. Fractional Cholesterol synthesis represents the fraction of free cholesterol in plasma that was newly synthesised.
Postprandial Fractional Cholic Acid Synthesis	baseline and 12 weeks	Changes from baseline in fractional cholic acid synthesis after 12 weeks of colesevelam or placebo treatment were evaluated. Fractional cholic acid synthesis represents the relative amount of cholic acid that is made from newly synthesised cholesterol.
Glucagon AUC	baseline and 12 weeks	Changes from baseline of glucagon AUC after 12 weeks of placebo or colesevelam treatment. AUC values were calculated by the trapezoid method using all results between 0 and 300 minutes

Trial Locations

Locations (3): Diablo Clinical Research, Inc
🇺🇸
Walnut Creek, California, United States
Clinical Pharmacology of Miami, Inc
🇺🇸
Miami, Florida, United States
Diabetes & Glandular Disease Research Associates
🇺🇸
San Antonio, Texas, United States