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Combination Therapy of F8IL10 and Methotrexate in Rheumatoid Arthritis Patients

Phase 1
Completed
Conditions
Rheumatoid Arthritis
Interventions
Drug: F8IL10
Drug: Methotrexate
Registration Number
NCT02076659
Lead Sponsor
Philogen S.p.A.
Brief Summary

Phase I, multicenter, open-label, dose escalation study to test the efficacy and safety of F8IL10 and methotrexate when given as a combination in rheumatoid arthritis patients.

Detailed Description

The study is designed to explore whether F8IL10 can be safely administered in combination with standard-dose of MTX in patients with active rheumatoid arthritis and to determine the recommended dose of F8IL10 when combined with MTX.

As soon as the MTD/RD is determined, an additional 12 patients will be randomized (6+6) between F8IL10 (RD) and placebo to further investigate the safety and pharmacacodynamics profile of the study treatment.

Methotrexate (MTX) will be administered as concomitant medication in the dose escalation as well as in the randomized part of the study.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
36
Inclusion Criteria

Not provided

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Exclusion Criteria

Not provided

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Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
F8IL10 + MTXF8IL10Ten cohorts of 3-6 RA patients will be treated at increasing doses per cohort of F8IL10 plus fixed doses of MTX and folic acid. An additional 12 patients will be randomized (6+6) in a double blind, placebo controlled cohort with F8IL10 given at RD and placebo. In both arms, MTX will be administered as concomitant medication. In all coohorts a stable dose of folic acid (5 mg) will be administered on Day 2.
F8IL10 + MTXMethotrexateTen cohorts of 3-6 RA patients will be treated at increasing doses per cohort of F8IL10 plus fixed doses of MTX and folic acid. An additional 12 patients will be randomized (6+6) in a double blind, placebo controlled cohort with F8IL10 given at RD and placebo. In both arms, MTX will be administered as concomitant medication. In all coohorts a stable dose of folic acid (5 mg) will be administered on Day 2.
Primary Outcome Measures
NameTimeMethod
Number of patients with adverse events that are related to treatment and classified as DLTs for each administered dosageUp to day 28

To establish the MTD and the RD of F8IL10 when administered in combination with methotrexate

Secondary Outcome Measures
NameTimeMethod
Accumulation ratio for Cmin [R min]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Response rate according to EULAR criteria (Good, Moderate and Non-responders) based on DAS28 score1) from day -14 up to day 0 (screening); 2) at day 1 of week 5; 3) at day 1 of week 9; 4) from week 9-13 up to week 57-61, every 4 weeks (safety/efficacy follow-up)

To explore the antiarthritic activity of the study medication in patients with active rheumatoid arthritis.

Terminal half-life [t1/2]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Area under the drug concentration-time curve [AUC(0 - t last)]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Maximum drug concentration [Cmax]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

ACR 20, ACR 50, ACR 70 response rate1) from day -14 up to day 0 (screening); 2) at day 1 of week 5; 3) at day 1 of week 9; 4) from week 9-13 up to week 57-61, every 4 weeks (safety/efficacy follow-up)

To explore the antiarthritic activity of the study medication in patients with active rheumatoid arthritis.

Area under the drug concentration-time curve, extrapolated to infinity [AUC]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Accumulation ratio for Cmax [Rmax]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Time to reach maximum drug concentration [Tmax]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Accumulation ratio for AUC [R AUC]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Relative change over time of blood biomarkersFrom day -14 up to day 0 (screening); at day 1 of week 1; at day 1 of week 5 /week 9 (EoT); from week 7 up to week 11 (safety follow-up); from week 11 up to week 15 (efficacy follow-up); from week 11-15 up to week 57-61, every 4 weeks (total follow-up)
Total clearance following the dose administered [CL]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Volume of distribution at steady state [Vss]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Mean residence time [MRT]At day 1, 4, 5, 6 of week 1; at day 1, 2, 3, 4, 5, 6 of week 4

Pharmacokinetics assessment of F8IL10 through blood sampling

Human anti-fusion protein antibodies (HAFA) levels1) at day 1 of week 1; 2) at day 1 of week 4; 3) from week 5 up to week 9 (EoT visit)

Investigate the potential induction of human anti-fusion protein antibodies (HAFA) through standard laboratory analysis.

Change from baseline in DAS281) from day -14 up to day 0 (screening); 2) at day 1 of week 5; 3) at day 1 of week 9; 4) from week 9-13 up to week 57-61, every 4 weeks (safety/efficacy follow-up)

To explore the antiarthritic activity of the study medication in patients with active rheumatoid arthritis.

Trial Locations

Locations (5)

Azienda Ospedaliera San Camillo-Forlanini Roma

🇮🇹

Roma, Italy

Policlinico San Matteo, Pavia

🇮🇹

Pavia, Italy

Pisa University Hospital

🇮🇹

Pisa, Italy

Siena University Hospital

🇮🇹

Siena, Italy

Policlinico A. Gemelli, Università Cattolica del Sacro Cuore

🇮🇹

Roma, Italy

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