Urinary Actin, as a Potential Marker of Sepsis-related Acute Kidney Injury
- Conditions
- Acute Kidney Injury Due to SepsisSepsis
- Interventions
- Other: Sepsis therapyOther: Sepsis-related acute kidney injury therapy
- Registration Number
- NCT04968262
- Lead Sponsor
- University of Pecs
- Brief Summary
In our study, 17 septic, 43 sepsis-related acute kidney injury and 24 control patients were enrolled. Blood and urine samples were collected at the intensive care unit from acutely diagnosed septic and sepsis-related acute kidney injury patients at three time points (T1-3): T1: within 24 hours after admission; T2: second day morning; T3: third day morning of follow-up. Patients with malignancies needing palliative care, end-stage renal disease or kidney transplantation were excluded. Not more than one sample (venous blood, midstream spot urine) was collected from control patients. Serum and urinary actin levels were determined by quantitative Western blot. Urinary actin concentrations were expressed as µg/L, while serum actin levels were expressed as mg/L. Data were compared with laboratory and clinical parameters. Patients were categorized by the Sepsis-3 definitions and 30-day mortality data were investigated.
- Detailed Description
Actin is a globular protein present in every cell with a 42 kilodalton molecular mass. It plays an important role in muscle contraction while being an essential component of the cytoskeleton as well. Several proteins (e.g. gelsolin, Gc-globulin) bind actin in the circulation during the physiological cell turnover, thus making its urinary appearance unlikely. However, recent studies indicate that actin could be detected in the urine of kidney-transplant patients with acute kidney injury. Therefore, the main focus of our research was the detection and measurement of actin in the blood and urine in patients with sepsis or sepsis-related acute kidney injury, as the early recognition of kidney injury - especially in sepsis - is essential in the aspect of therapy and survival.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 84
- Sepsis
- Sepsis-related acute kidney injury
- malignancies needing palliative care
- end-stage renal disease
- kidney transplantation
- under 18 years of age
- unobtainable consent
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Sepsis Sepsis therapy Patients receive sepsis therapy. Sepsis-related acute kidney injury Sepsis-related acute kidney injury therapy Patients receive sepsis and sepsis-related acute kidney injury therapy.
- Primary Outcome Measures
Name Time Method Serum actin concentrations 3 days Clotted blood samples were centrifuged (10 min, 1500 g) and supernatants were treated with electrophoresis sample buffer then heated at 100 °C for 5 minutes. Serum actin was determined by quantitative Western blot. Native and denatured sample aliquots were stored at -70 °C until analysis.
Urinary actin concentrations 3 days Urine samples were centrifuged (10 min, 1500 g) and supernatants were treated with electrophoresis sample buffer then heated at 100 °C for 5 minutes. Urinary actin was determined by quantitative Western blot. Native and denatured sample aliquots were stored at -70 °C until analysis.
- Secondary Outcome Measures
Name Time Method U-actin/u-creatinine concentrations 5 days U-actin values were determined by quantitative Western blot, while U-creatinine concentrations were measured using automated routine laboratory procedures, therefore U-actin/u-creatinine ratios could be calculated.
Trial Locations
- Locations (1)
Department of Anesthesiology and Intensive Therapy, Medical School, University of Pécs
🇭🇺Pécs, Baranya, Hungary