Mechanisms of Acute Kidney Injury in Severe Infections
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Sepsis
- Sponsor
- Uppsala University Hospital
- Enrollment
- 15
- Locations
- 1
- Primary Endpoint
- Presence of neutrophil elastase in the kidneys in patients with sepsis and acute kidney injury (AKI) by 11C-GW457427 measured with dynamic positron emission tomography (PET)
- Status
- Recruiting
- Last Updated
- last year
Overview
Brief Summary
Renal perfusion and neutrophil-mediated inflammation will be assessed in the kidney in sepsis patients with acute kidney injury using positron emission tomography. For marked water will be used for renal perfusion and a newly developed PET tracer molecule (11C-GW457427) with specific binding to neutrophil elastase which provides a measure of the amount of infiltrating neutrophils in the renal parenchyma for inflammation. The study is performed in a PET-CT camera where anatomical imaging takes place at the same time as the PET examinations.
Detailed Description
To study renal perfusion and neutrophil-mediated inflammation 10 critically ill patients with sepsis and acute kidney injury (AKI) and 5 healthy volunteers as controls will be recruited and examined at one time with marked water followed by neutrophil elastase tracer. At the same time, a CT scan is performed for anatomy. Plasma levels of inflammatory mediators will be measured. Clinical data will be collected. The endpoints are changes in renal perfusion and increased presence of neutrophil elastase in the renal parenchyma.
Investigators
Miklos Lipcsey
Professor
Uppsala University Hospital
Eligibility Criteria
Inclusion Criteria
- •Patients with sepsis and AKI
- •\> 30 years
- •At least AKI stage 1 according to KDIGO where the last plasma creatinine is measured within 24 hours of the examination
- •Healthy volunteers
- •\> 30 years
- •No previously known kidney disease
- •Normal plasma creatinine value
Exclusion Criteria
- •Chronic renal failure (CKD stage \>3a) or dialysis
- •Instability in vital functions that makes PET-CT examination unsuitable
- •Claustrophobia or other reasons that make the patient unable to lie still during the examination.
- •Pregnancy and breastfeeding
Outcomes
Primary Outcomes
Presence of neutrophil elastase in the kidneys in patients with sepsis and acute kidney injury (AKI) by 11C-GW457427 measured with dynamic positron emission tomography (PET)
Time Frame: PET performed within days of AKI
vs controls
Renal perfusion in patients with sepsis and acute kidney injury (AKI) measured by 15O-labeled water in dynamic positron emission tomography (PET)
Time Frame: PET performed within 10 days of AKI
vs controls