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A Phase 1 clinical trial to evaluate safety and effectiveness of ZIKA vaccine in healthy adults.

Phase 1
Completed
Registration Number
CTRI/2017/05/008539
Lead Sponsor
Bharat Biotech International Ltd
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
Not specified
Target Recruitment
48
Inclusion Criteria

1 Normal healthy male and female volunteers aged between 18 and 65 years weighing at least 50kgs of body weight

2 Ability to comprehend the full nature and purpose of the study, including possible risks and adverse events; ability to co-operate with the Investigator and to comply with the requirements of the entire study

3 Signed written informed consent prior to inclusion in the study

4 Seronegative for Zika by ELISA

5 Dengue sero-negative at baseline by screening laboratory evaluation, confirmed by Dengue IgG by ELISA method for Group 1 participants

6 Dengue seropositive at baseline by screening laboratory evaluation, confirmed by Dengue IgG by ELISA method for Group 2 participants

7 Dengue vaccination or suffered from Dengue viral fever for Group 2 volunteers

8 No history of yellow fever vaccination

9 No history of vaccination to Japanese encephalitis vaccination

10 Since active (live) ZIKV infection is known to cause teratogenicity, women of child-bearing potential should agree to use medically effective contraception (oral contraception, barrier methods, spermicide, etc.), preferably double contraception or have a partner who is sterile from enrollment to 3 months following the last injection, or have a male partner who is medically unable to induce pregnancy.

11 Sexually active men who are considered sexually fertile must agree to use either a barrier method of contraception, preferably double contraception during the study, and agree to continue the use for at least 3 months following the last injection, or have a partner who is permanently sterile or is medically unable to become pregnant.

12 A negative urine or serum pregnancy test before administration of investigational vaccine on day of screening (Serum Pregnancy Test), and Day 0 and Day 28 (both days Urine Pregnancy Test)

13 No history of clinically significant immunosuppressive or autoimmune disease.

14 Laboratory investigations must be within normal limits

a)Hemoglobin >=10gm/dL

b)WBC (white blood cells) >=4000/mm3

c)Platelets >=100,000/mm3

d)Bilirubin and AST/ ALT <1.5 x ULN (upper limit of normal)

e)Creatinine <1.5 x ULN for the clinical laboratory

15 Not currently or within the previous 4 weeks taking immunosuppressive agents (excluding inhaled, topical skin and/or eye drop-containing corticosteroids, low-dose methotrexate, or corticosteroids at a dose less than 20 mg/day).

16 Patients should be otherwise healthy as determined by physical examination, medical history, and no significant abnormality in any of the clinical parameters including ECG and Chest X-ray.

17 Willing to allow storage and future use of biological samples for Zika virus related research

Exclusion Criteria

1 Administration of an investigational vaccine or drug either currently or within 30 days of first BBV121 vaccination

2 Previous receipt of an investigational vaccine or drug for the treatment or prevention of Zika virus

3 Administration of any vaccine within 4 weeks of first dose

4 Administration of any monoclonal or polyclonal antibody product within 4 weeks of the first dose of BBV121 vaccination

5 Administration of any blood product within 3 months of first dose

6 Pregnancy or breast feeding or plans to become pregnant during the study

7 Positive serologic test for HIV, hepatitis B surface antigen (HBsAg); or any potentially communicable infectious disease as determined by the Principal Investigator or Medical Monitor

8 Positive serologic test for hepatitis C (exception: successful treatment with confirmation of sustained virologic response);

9 Chronic liver disease or cirrhosis

10 Immunosuppressive illness including hematologic malignancy, history of solid organ or bone marrow transplantation

11 Current or anticipated concomitant immunosuppressive therapy (excluding inhaled, topical skin and/or eye drop-containing corticosteroids, low-dose methotrexate, or corticosteroids at a dose less than 20 mg/day)

12 Current or anticipated treatment with TNF-α inhibitors such as infliximab, adalimumab, and etanercept

13 Prior major surgery or any radiation therapy within 4 weeks of enrolment

14 Any pre-excitation syndromes, e.g., Wolff-Parkinson-White syndrome

15 Presence of a cardiac pacemaker or automatic implantable cardioverter defibrillator

16 Metal implants within 20 cm of the planned site(s) of injection

17 Presence of keloid scar formation or hypertrophic scar at the planned site(s) of injection

18 Prisoner or participants who are compulsorily detained (involuntary incarceration) for treatment of either a physical or psychiatric illness

19 Active addictive drug or alcohol use or dependence that, in the opinion of the investigator, would interfere with adherence to study requirements or assessment of immunologic endpoints

20 Blood donations/ losses within 2 months of screening

21 Significant orthostatic hypotension (i.e., a drop in systolic blood pressure of 30 mm Hg or more and/or a drop in diastolic blood pressure of 20 mmHg or more on standing)

22 Prior radiotherapy in 30 days or less

23 Significant pre-existing co-morbidities

i Cardiovascular

Myocardial infarction within the last 6 months

Congestive heart failure

Unstable angina

Active cardiomyopathy

Cardiac arrhythmia

Uncontrolled hypertension

History of familial long QT syndrome or sudden cardiac death

ii Pulmonary disease requiring oxygen

iii Neurologic and psychiatric

History of significant neurologic or psychiatric disorder that would preclude study compliance or ability to give informed consent

iv Rheumatic arthralgia

24 Participants not having adequate hematologic reserve

i Hemoglobin <=10gm/dL

ii WBC (white blood cells) <=4000/mm3

iii ANC (absolute neutrophils count) <=2000/ mm3

iv Platelets <=100,000/mm3

25 Inadequate hepatic function at screening as defined by:

i Bilirubin >1.5 x ULN (upper limit of normal)

ii AST/ ALT >1.5 x ULN

26 Inadequate renal function at screeni

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
To evaluate the safety and tolerability of two-doses of three-sequentially escalating cohort (2.5 µg, 5 µg and 10 µg) of BBV121 (inactivated Zika virus vaccine) compared with Placebo (Alum). <br/ ><br>�Mean change from baseline in safety laboratory parameters <br/ ><br>�Incidence of solicited AEs post-vaccination <br/ ><br>�Incidence of unsolicited AEs post-vaccination <br/ ><br>�Incidence of SAE <br/ ><br>Timepoint: day0,day28,day56,day 180,day270 and day360
Secondary Outcome Measures
NameTimeMethod
To evaluate the magnitude and frequency of ZIKV-specific neutralizing antibodies as measured by PRNT50 methodTimepoint: day0,day28,day56,day 180,day270 and day360
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