The Effect of Clopidogrel and Ticagrelor With and Without Acetylsalicylic Acid (ASA) on Hemostatic System Activation at the Site of Plug Formation in Vivo in Man

Phase 2

Completed

• Conditions: Acute Coronary Syndrome
• Interventions: Drug: Ticagrelor; Drug: Clopidogrel; Drug: Placebo; Drug: ASA

• Registration Number: NCT02120092
• Lead Sponsor: Medical University of Vienna

Brief Summary

Background: Coronary heart disease is the most common cause of death in industrialized countries. Revascularisation by percutaneous coronary angioplasty or thrombolysis is the main principle for treatment of the acute coronary syndrome. To inhibit platelet activity patients are routinely given acetylsalicylic acid (ASA) and clopidogrel, a second-generation thienopyridine. Recently, ticagrelor, a novel cyclopentyl-triazolo-pyrimidine with several pharmacological advantages, has demonstrated greater efficacy but a higher bleeding risk than clopidogrel. Coronary thrombus formation is a complex process and the antithrombotic mechanisms of platelet function inhibitors are incompletely understood. Studies in venous blood or in vitro do not truly reflect the in vivo circumstances as they often do not take into account flow conditions or the interaction between endothelium, blood cells and coagulation factors. Results from animal models may not be relevant for the prothrombotic mechanisms in humans. We have developed a technique that allows investigating hemostatic system activation directly at the site of thrombus formation in vivo in humans.

Aim: to compare the inhibitory effects of clopidogrel and ticagrelor (with and without concomitant ASA) on hemostatic system activation under circumstances close to the in vivo situation.

Design, patients and interventions: prospective, randomized, double-blind, placebo controlled parallel-group study with a 2x2 factorial design including 112 healthy volunteers who will be randomised to 4 treatment arms: ticagrelor or clopidogrel + placebo, ticagrelor or clopidogrel + ASA.

Outcome variables: Indicators of platelet and coagulation activation \[ß-thromboglobulin and thromboxane B2 as well as prothrombin fragment F1+2 and D-Dimer, respectively\] will be measured before and at several time points during a 8 day period in venous blood and in blood emerging from a standardized injury of the microvasculature to determine bleeding time (shed blood).

Statistical considerations: Sample size calculation is based on the percent change in the main outcome variable "β-TG in shed blood" from baseline to 2 hours after treatment start. Statistical analysis is based on the full analysis set, including all randomized subjects who received at least the starting dose of the study medication and for whom blood collections at baseline and at 2 hours after treatment start have been performed.

Detailed Description

Not available

Study Timeline

• Study Start: 2010-10
• Primary Completion: 2013-12
• Study Completion: 2013-12
• Status Verified: 2014-04
• Study First Submitted: 2014-04-17
• Study First Submitted QC: 2014-04-17
• Last Update Submitted: 2014-04-17
• Study First Posted: 2014-04-22
• Last Update Posted: 2014-04-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 89

Inclusion Criteria

• young, healthy males

Exclusion Criteria

• history of bleeding
• any medication
• known intolerance to study drug(s)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Clopidogrel + Placebo	Placebo	-
Ticagrelor + ASA	Ticagrelor	-
Ticagrelor + Placebo	Placebo	-
Clopidogrel + ASA	ASA	-
Clopidogrel + ASA	Clopidogrel	-
Clopidogrel + Placebo	Clopidogrel	-
Ticagrelor + Placebo	Ticagrelor	-
Ticagrelor + ASA	ASA	-

Primary Outcome Measures

Name	Time	Method
ß-Thromboglobulin in shed blood 2h after first study drug intake	2 hours after first study drug intake

Trial Locations

Locations (1)

Medical University of Vienna; 🇦🇹 Vienna, Austria