The Impact of Dietary Branched-chain Amino Acid (BCAA) Restriction on the Development of Insulin Resistance and Anabolic Resistance During Forearm Immobilization in Healthy, Young Volunteers.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Forearm glucose uptake
研究概览
简要总结
The present study will investigate the impact of dietary branched chain amino acid restriction on muscle atrophy, insulin sensitivity and muscle protein synthesis following short-term forearm immobilization
详细描述
Twenty-four healthy young volunteers will undergo 2 days of forearm immobilization in combination with either a branched chain amino acid restricted diet or a control diet. Before and after immobilization, they will receive a stable isotope tracer infusion (5.5 h) combined with repeated blood and muscle sampling under insulin clamp conditions, in order to measure insulin sensitivity and muscle protein synthesis in the fasted and fed state.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Investigator)
盲法说明
Double blinded
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males and females 18-40 years of age
- •Body mass index between 18.5 and 30 kg/m2
排除标准
- •Any diagnosed metabolic impairment (e.g. type 1 or 2 diabetes)
- •Any diagnosed cardiovascular disease
- •Hypertension (≥140 mmHg systolic and/or ≥90 mmHg diastolic)
- •Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices)
- •Regular use of nutritional supplements
- •Currently involved in a structured progressive resistance training programme (>3 times per week)
- •A personal or family history of thrombosis, epilepsy, seizures or schizophrenia
- •Any previous motor disorders or disorders in muscle and/or lipid metabolism
- •Known hypokalaemia
- •Presence of an ulcer in the stomach or gut and/or strong history of indigestion
- •Known severe kidney problems
- •Pregnant or breastfeeding
- •Unable to give consent
结局指标
主要结局
Forearm glucose uptake
时间窗: Hyperinsulinaemic-euglycaemic clamps are performed before and immediately after 2 days of forearm immobilisation. Forearm glucose uptake is measured during the steady state period of the clamp.
Insulin sensitivity, measured as forearm glucose uptake, under hyperinsulinaemic-euglycaemic conditions
次要结局
- Muscle protein synthesis rates(During 3 hours of hyperinsulinaemic-euglycaemic clamped conditions before and after 2 days of immobilisation)
- Intramuscular BCAA concentration(Before and after 3 hours of hyperinsulinaemic-euglycaemic clamped conditions)
- BCAA concentrations between forearm arterialized venous and deep venous blood(Fasted and during the steady-state phase of the insulin clamp (i.e. last 30 minutes))
- Whole-body substrate oxidation(Hyperinsulinaemic-euglycaemic clamps are performed before and immediately after 2 days of forearm immobilisation. Whole-body substrate oxidation is measured before the commencement of, and during the steady state period of the clamp (last 30 minutes))