Liquid Protein Preloads With Different Carbohydrate Types Effects on the Glycaemic and Insulinaemic Response

Not Applicable

Completed

• Conditions: Diabetes; Impaired Glucose Tolerance; Obesity
• Interventions: Dietary Supplement: Bread, Soy milk and cow milk; Device: real-time ultrasonography (GE LOGIQ P5 ultrasound in CNRC)

• Registration Number: NCT02151188
• Lead Sponsor: Clinical Nutrition Research Centre, Singapore

Brief Summary

This study hypothesizes that milk protein consumed together or shortly before a white rice or white bread carbohydrate meal exerts different influence on the glycaemic, insulinaemic responses.

Detailed Description

Consumption of milk proteins increase satiety and reduce glucose response when consumed alone or with carbohydrate. Milk proteins are of interest because proteins are more satiating than either carbohydrate or fat, and they regulate food intake and metabolic functions by the intact protein, encrypted peptides and amino acids on gastrointestinal and central pathways. White rice and white bread are two common carbohydrates types mostly consumed in Asia. Both of them are known to result in high glycaemic and insulinaemic responses. However, in most meals, they are accompanied by other foods such as soy milk, cow milk products. The glyceamic and insulinaemic responses are determined by the type of protein and the type of carbohydrates. And also the consumption time of protein and carbohydrate. Recent research points to an intrinsic value of small amounts of milk protein or dairy consumed shortly before a meal can reduce the glycemic response to carbohydrate and that this is not at the cost of increased demand for insulin. The objective of the present study is to investigate how glycaemic, insulinaemic and satiety responses to a white rice or white bread meal changed when a soy milk or cow milk consumed together or shortly before the meal. Whole soy milk and whole cow milk will be added to the meal. Healthy subjects (n 15) will ingest the test meals once. The meals will be provided as breakfasts, on 10 different occasions, in random order with ≥ 7 days between each. Blood samples (from the cannula and finger pricks) will be then drawn for 3 h, and glucose and serum insulin, plasma glucagon, satiety markers, inflammation markers, amino acids et.al will be analysed. The objective of the study is determining if different liquid protein consumed shortly before or with carbohydrate has different effects on metabolism. And also we will compare the difference of white rice and white bread. Determining the consuming time effect, milk protein type and carbohydrate type on glycaemic and insulinaemic are critical for the provision of dietary advice and formulation of public health guidelines.

Study Timeline

• Study Start: 2014-03
• Study First Submitted: 2014-05-28
• Study First Submitted QC: 2014-05-29
• Study First Posted: 2014-05-30
• Primary Completion: 2015-10
• Study Completion: 2015-10
• Status Verified: 2016-02
• Last Update Submitted: 2016-02-01
• Last Update Posted: 2016-02-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 15

Inclusion Criteria

• Chinese ethnicity
• Age between 21-40 years
• Not on prescription medication known to affect glucose and satiety related metabolism
• Do not partake in sports at the competitive and/or endurance levels
• Weight of at least 45kg
• Body mass index between 18.0 to 24.9 kg/m2
• Normal blood pressure (120/80 mmHg)

Exclusion Criteria

Smoker

• fasting blood glucose>5.5 mmol/L
• Any major medical conditions including diabetes, hypertension, cardiovascular disease, thyroid disorders
• Allergic or intolerant to foods presented in the study

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Bread and water	Bread, Soy milk and cow milk	co-ingestion control session
bread with soy milk co-ingestion	Bread, Soy milk and cow milk	co-ingestion bread with soy milk
bread with cow milk co-ingestion	Bread, Soy milk and cow milk	co-ingestion bread with cow milk
preload soy milk	Bread, Soy milk and cow milk	preload soy milk 30 min, then bread
Bread and water	real-time ultrasonography (GE LOGIQ P5 ultrasound in CNRC)	co-ingestion control session
preload cow milk	real-time ultrasonography (GE LOGIQ P5 ultrasound in CNRC)	preload cow milk 30 min, then bread
bread with cow milk co-ingestion	real-time ultrasonography (GE LOGIQ P5 ultrasound in CNRC)	co-ingestion bread with cow milk
bread with soy milk co-ingestion	real-time ultrasonography (GE LOGIQ P5 ultrasound in CNRC)	co-ingestion bread with soy milk
preload cow milk	Bread, Soy milk and cow milk	preload cow milk 30 min, then bread
preload soy milk	real-time ultrasonography (GE LOGIQ P5 ultrasound in CNRC)	preload soy milk 30 min, then bread

Primary Outcome Measures

Name	Time	Method
Glucose response of different treatments	3.5 hrs post consumption	The blood glucose will be measured by Hemocue 201 from finger prick. Venous blood will be collected at the same time points.

Secondary Outcome Measures

Name	Time	Method
Gastric emptying rate	2 hours post consumption	Measurement of gastric emptying rate using real-time ultrasonography (GE LOGIQ P5 ultrasound in CNRC). Ultrasound measurements will be taken with subjects in a supine position during fasting, then at 15-30 min intervals after consumption of the test meal.

Trial Locations

Locations (1)

Clinical Nutrition Research Centre; 🇸🇬 Singapore, Singapore