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Oncotype DX in BRCA-mutated Breast Cancer Patients: Our Experience. A Retrospective Observational Monocentric Study

Completed
Conditions
Genetic Predisposition
Breast Cancer
Interventions
Genetic: Oncotype DX assay
Registration Number
NCT06484725
Lead Sponsor
IRCCS San Raffaele
Brief Summary

The study aims to evaluate the distribution of the Oncotype DX RS® genomic test in patients with ER-positive and HER2-negative breast cancer treated from January 2012 to December 2021, focusing on patients presenting germline mutations.

Detailed Description

In the past decades, genetic testing for cancer susceptibility has become essential in breast cancer (BC) management. Up to 10% of BC cases are associated with an inherited mutation in various genes, especially BRCA 1 and BRCA 2. Estrogen receptor (ER) positivity is observed in 22% on BRCA 1 and 77% of BRCA 2 mutation carrier; moreover, other genes are strongly related to ER-positive cancer.

Oncotype DX assay (Genomic Health, Inc, Redwood City, Calif) is a 21 gene panel developed to predict the risk of tumor recurrence in patients with ER-positive and human epidermal growth factor 2 (HER2)- negative BC; nowadays, this test represents a standard of care to guide adjuvant chemotherapy decision recommended in several guidelines. According to Oncotype DX Recurrent Score (RS)® a high RS shows a worse prognosis and predicts chemotherapy benefit in patients with ER-positive HER2-negative BC.

Combining Oncotype DX RS® with other risk factor elements, in particular germline mutations, could help to select patients who would benefit from an appropriate adjuvant therapy.

This retrospective observational study aims to evaluate Oncotype DX RS® distribution in ER-positive HER2-negative BC patients treated from January 2012 to December 2021, focusing on patients harboring mutation in BC-associated genes compared to the general population.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
250
Inclusion Criteria
  • Age at diagnosis at least 18 years
  • Women who underwent breast surgery in OSR
  • Patients with ER-positive, HER2-unamplified early-stage BC who underwent the Oncotype DX assay, according to the standard of care.
Exclusion Criteria
  • Male
  • ER negative or HER2 amplified disease
  • Patient s who did not undergo breast surgery in OSR
  • Patient s who did not undergo the Oncotype Dx assay

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
VUS patientsOncotype DX assayPatients with VUS in Breast Cancer-related genes candidated to surgery as primary treatment and eligible for Oncotype DX assay.
Wild type patientsOncotype DX assayPatients with no mutations or VUS in Breast Cancer-related genes candidated to surgery as primary treatment and eligible for Oncotype DX assay.
Mutated patientsOncotype DX assayPatients with mutation in Breast Cancer-related genes candidated to surgery as primary treatment and eligible for Oncotype DX assay.
Primary Outcome Measures
NameTimeMethod
Oncotype DX assay and RS® in participants.30 days after surgery.

To evaluate the Oncotype DX RS® distribution in ER-positive early BC patients comparing patients with a deleterious germline mutation, patients with a VUS in BC-related genes and patients with no mutation.

Secondary Outcome Measures
NameTimeMethod
Distant or local recurrence.Up to 10 years after surgery

Evaluation of distant or local recurrence from follow-up data

Oncotype DX assay and RS® in participants by menopausal or nodal status.30 days after surgery

To evaluate Oncotype DX RS® distribution stratifying patients by menopausal or nodal status.

Trial Locations

Locations (1)

IRCCS Ospedale San Raffaele

🇮🇹

Milan, MI, Italy

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