Effect of Amino Acid Addition to a Drink on the Appearance of Ingested Water in Body Fluids1
- Conditions
- Rate of Water Appearance/Delivery in the Blood Water Balance
- Interventions
- Other: Composition of Sports Drink
- Registration Number
- NCT04819334
- Lead Sponsor
- Loughborough University
- Brief Summary
To determine the effects of a moderate amount of amino acids on the rate of water absorption and availability as a precursor fluid for sweat. Young and healthy (male or female) volunteers will take part in three experimental trials. In each trial, volunteers will be given one of two commercially available sports drinks or a commercially available sport drink with added amino acids in a double-blinded, randomised, crossover design. Each drink will be a single 600 mL bolus. All beverages will be labelled with deuterium (D2O). Trials will be compared for temporal accumulation of deuterium in plasma over the course of 60 minutes. Whole blood measurements of haemoglobin and haematocrit will also be made and plasma volume changes calculated. It is hypothesised that the amino acid trial will increase the rate of fluid absorption.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 15
- Generally fit and healthy (determined by health screen questionnaire)
- Recreationally active (minimum 3 hours of physical activity per week including walking)
- Any cardiovascular, gastrointestinal, renal or acute/chronic health conditions that may influence the outcomes
- Smoking (including vaping)
- BMI of greater than 30 (combined with a body fat percentage greater than 20%) or BMI lower than 17.5
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Commercially Available Sports Drink A Composition of Sports Drink A commercially available flavored electrolyte solution, The Coca-Cola Company Commercially Available Sports Drink A with added Amino Acids Composition of Sports Drink he same as sports drink A above (a commercially available flavored electrolyte solution, The Coca-Cola Company), but with the addition of a small amount of amino acids (\~2.6 g/100 ml). Commercially Available Sports Drink B Composition of Sports Drink A commercially available flavored electrolyte solution, PepsiCo
- Primary Outcome Measures
Name Time Method Time at which 50% of drink was absorbed (t1/2) 60 minutes Determined from D2O values in venous blood samples collected before and after drink ingestion
Maximum absorption rate of drink 60 minutes Determined from D2O values in venous blood samples collected before and after drink ingestion
Time at which drink absorption rate returned to 0 (t2) 60 minutes Determined from D2O values in venous blood samples collected before and after drink ingestion
Area under the curve for D2O 60 minutes Determined from D2O values in venous blood samples collected before and after drink ingestion
Time at which drink absorption rate was maximum (t1) 60 minutes Determined from D2O values in venous blood samples collected before and after drink ingestion
- Secondary Outcome Measures
Name Time Method Urinary specific gravity 60 minutes Determined from urine samples collected before and after drink ingestion
Plasma volume 60 minutes Determined from haemoglobin and haematocrit measures in venous blood samples collected before and after drink ingestion
Plasma osmolality 60 minutes Determined from venous blood samples collected before and after drink ingestion
Plasma lactate concentration 60 minutes Determined from venous blood samples collected before and after drink ingestion
Plasma creatinine concentration 60 minutes Determined from venous blood samples collected before and after drink ingestion
Plasma amino acid concentration 60 minutes Determined from venous blood samples collected before and after drink ingestion
Plasma glucose concentration 60 minutes Determined from venous blood samples collected before and after drink ingestion
Trial Locations
- Locations (1)
Loughborough University
🇬🇧Loughborough, Leicestershire, United Kingdom