Acute Effects of Breaking Prolonged Sitting on Postprandial Glycaemic Response, Incretin Hormones Secretion and Eating Behaviour in Sedentary Lean Adults
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Enrollment
- 13
- Locations
- 1
- Primary Endpoint
- Gut hormones responses
Study Overview
Brief Summary
The purpose of this project is to investigate the effects of breaking up prolonged sitting on postprandial metabolic responses, gut hormones secretion and energy balance in sedentary lean adults.
Detailed Description
Two trials are included - Prolonged sitting and Breaking sitting.
In the Prolonged sitting trial, after consuming breakfast, participants sat on a chair for 180 min. After 180 min, lunch was provided. After finishing lunch, participants sat on a chair for another 120 min. In the Breaking sitting trial, after consuming breakfast, participants walked 2 min on a treadmill every 20 minutes for the following 180 min. For the remainder of the time participants sat on the chair. After 180 min, lunch was provided. After finishing lunch, participants continued 2 min of walking every 20 min for the following 120 min. Blood samples were collected in a regular pattern throughout the trials. Energy balance (e.g., energy intake and spontaneous physical activity) was assessed using food diary and an accelerometer until midnight in the trial days once participants left laboratory.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 20 Years to 45 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Physical inactive males and females
- •Aged between 20 and 45 years
- •Lean healthy (waist circumference less than 90 cm for males and 80 cm for females)
- •Weight stable for more than 3 months (no change in weight +/- 3%)
- •Non-smoker
- •Able to walk comfortably on a treadmill
Exclusion Criteria
- •Personal history of/existing diabetes, cardiovascular disease, metabolic disease or dyslipidaemia
- •Waist circumference > 90 cm for male and > 80 cm for female
- •Taking medications that may influence lipid or carbohydrate metabolism or immune system function
- •Unable to take part in exercise for any reason (e.g., injury or disability) or a positive response to any questions on the Physical Activity Readiness questionnaire (PAR-Q)
Arms & Interventions
Prolonged sitting
Participants sat on a chair during the trial.
Intervention: Prolonged sitting (Behavioral)
Breaking sitting
Participants walked regularly during the trial.
Intervention: Breaking sitting (Behavioral)
Outcomes
Primary Outcomes
Gut hormones responses
Time Frame: 320 minutes
Changes in blood gut hormones responses (e.g., GLP-1, PYY and GIP concentrations measured in pmol/L) between trials
Secondary Outcomes
- Visual analogue scale (VAS)(320 minutes)
- Physical activity level(600 minutes after leaving the laboratory)
- Blood pressure(320 minutes)
- Computerized cognitive testing(320 minutes)
- Energy intake(600 minutes after leaving the laboratory)
- Lipid profile(320 minutes)
- Incremental area under curve (iAUC) for metabolic health(320 minutes)
Investigators
Yung-Chih Chen
Principal Investigator
National Taiwan Normal University