Growth Factors Supplementation to Single-Step Culture Medium
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 426
- 试验地点
- 1
- 主要终点
- Clinical Pregnancy rate ( % )
研究概览
简要总结
The investigators suppose that adding growth factors that had been found in the female tubal fluid and uterus to In Vitro single step culture media for human embryos might improve the the embryogenesis In Vitro and the clinical outcome of the resulting off spring.
详细描述
The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding growth factors ( Insulin-like growth factor I, Insulin-like growth factor II, transforming growth factor-beta and epidermal growth factor ) as a promoting growth factors to Lifeglobal culture media might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between to arm of the same culture media and the same culture condition but with one arm with growth factors and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •all patients
排除标准
- •over 40 years old
研究组 & 干预措施
Culture media with growth factors and Cytokine
Growth factors and Cytokine adding to culture media to detect the deference and monitor the embryological outcome.
干预措施: Growth Factors and Cytokine adding to embryo culture media (Other)
In Vitro culture media
culture media to monitor the embryological outcome as control group
干预措施: No Intervention (Other)
结局指标
主要结局
Clinical Pregnancy rate ( % )
时间窗: 3 months
次要结局
- ongoing Pregnancy rate ( % )(6 months)
- rates of blastocyst formation and quality(6 days of culture)
研究者
Muhammad Fawzy
IVF Lab Director
Ibn Sina Hospital