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Evaluation of IFNγ and Inflammatory Mediators in Patients With Hemophagocytic Lymphohistiocytosis

Completed
Conditions
Hemophagocytic Lymphohistiocytoses
Interventions
Other: Blood Draws Data Collection
Registration Number
NCT06047210
Lead Sponsor
Swedish Orphan Biovitrum
Brief Summary

This observational study is designed to provide a better understanding of the potential use of interferon gamma (IFNγ) and IFNγ-inducible chemokines as markers of HLH disease activity in adults, and the potential of IFNγ to represent a therapeutic target.

Detailed Description

This is a observational study designed to determine the levels of pro-inflammatory markers in adult patients diagnosed with HLH and to assess the relationship between the biomarkers and disease activity in these patients during the HLH course. Inflammatory markers include IFNγ and IFNγ-induced chemokines C-X-C chemokine ligand 9 and C-X-C chemokine ligand 10.

In addition to the blood samples for the biomarker analysis, relevant information gathered by the treating physician will be collected in a data collection form. Whenever possible, collection of serum samples for biomarker analysis and relevant information should occur at HLH diagnosis, at regular time intervals during the treatment course (not more than once a week) up to resolution of HLH.

Blood samples will also be sent to the study sponsor (NovImmune S.A.) for cytokine testing. Before patient's samples are sent to the sponsor for testing, patient's name and any personal identifying information will be coded to protect participant's privacy.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
14
Inclusion Criteria
  • Patients >=18 years old diagnosed with active HLH as established by the treating physician.
  • HLH forms of unknown origin or secondary to infections or rheumatologic disorders.
  • Should the diagnosis of primary HLH or malignancy become apparent after inclusion, the data collected will be analyzed separately as additional cohorts.
  • The patient must have consented to the use of their clinical data for research purposes at the site.
Exclusion Criteria
  • Patients with primary HLH (diagnosed by the presence of homozygous mutations in a known HLH causative gene) and secondary HLH due to malignancy are excluded.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
A-HLH PatientsBlood Draws Data CollectionPatients diagnosed with A-HLH.
Malignancy-Associated Hemophagocytic Lymphohistiocytosis (M-HLH) PatientsBlood Draws Data CollectionPatients diagnosed with HLH in the context of a malignancy.
Primary Outcome Measures
NameTimeMethod
Mean Serum Concentration of Inflammatory Biomarker - C-X-C Chemokine Ligand 10 (CXCL10)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of CXCL10.

Mean Serum Concentration of Inflammatory Biomarker - C-X-C Chemokine Ligand 9 (CXCL9)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of CXCL9.

Mean Serum Concentration of Inflammatory Biomarker - Interferon Gamma (IFNg)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of IFNg.

Mean Serum Concentration of Inflammatory Biomarker - Interleukin 10 (IL-10)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of IL-10.

Mean Serum Concentration of Inflammatory Biomarker - Interleukin 1 Beta (IL-1B)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of IL-1B.

Mean Serum Concentration of Inflammatory Biomarker - Interleukin 6 (IL-6)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of IL-6.

Mean Serum Concentration of Inflammatory Biomarker - Neopterin11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of neopterin.

Mean Serum Concentration of Inflammatory Biomarker - Tumor Necrosis Factor Alpha (TNFa)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of TNFa.

Mean Serum Concentration of Inflammatory Biomarker - Total Human Interferon Gamma (hIFNg)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of hIFNg.

Mean Serum Concentration of Inflammatory Biomarker - Soluble CD163 (sCD163)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of sCD163.

Mean Serum Concentration of Inflammatory Biomarker - Soluble IL-2 Receptor Alpha (sIL2Ra)11 months

Serum samples was collected at various times after HLH diagnosis to determine the concentration of sIL2Ra.

Mean Serum Concentration of White Blood Cells11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of white blood cells.

Mean Serum Concentration of Red Blood Cells11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of red blood cells.

Mean Serum Concentration of Hemoglobin11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of hemoglobin.

Mean Serum Concentration of Hematocrit11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of hematocrit.

Mean Serum Concentration of Platelets11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of platelets.

Mean Serum Concentration of Neutrophils11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of neutrophils.

Mean Serum Concentration of Lymphocytes11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of lymphocytes.

Mean Serum Concentration of Monocytes11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of monocytes.

Mean Serum Concentration of Eosinophils11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of eosinophils.

Mean Serum Concentration of Basophils11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of basophils.

Mean Plasma Concentration of D-Dimer11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of D-dimer.

Mean Plasma Concentration of Fibrinogen11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of fibrinogen.

Mean Activated Partial Thromboplastin Time (aPTT)11 months

Blood samples was collected at various times after HLH diagnosis to determine the aPTT.

Mean Prothrombin Time11 months

Blood samples was collected at various times after HLH diagnosis to determine the prothrombin time.

Mean Serum Concentration of C-Reactive Protein11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of c-reactive protein.

Mean Serum Concentration of Ferritin11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of ferritin.

Mean Plasma Concentration of Blood Urea Nitrogen11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of blood urea nitrogen.

Mean Serum Concentration of Creatinine11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of creatinine.

Mean Serum Concentration of Albumin11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of albumin.

Mean Serum Concentration of Sodium11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of sodium.

Mean Serum Concentration of Fasting Triglycerides11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of fasting triglycerides.

Mean Serum Concentration of Total Bilirubin11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of total bilirubin.

Mean Serum Concentration of Conjugated Bilirubin11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of conjugated bilirubin.

Mean Serum Concentration of Alanine Aminotransferase11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of alanine aminotransferase.

Mean Serum Concentration of Aspartate Aminotransferase11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of aspartate aminotransferase.

Mean Serum Concentration of Alkaline Phosphatase11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of alkaline phosphatase.

Mean Serum Concentration of Lactate Dehydrogenase11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of lactate dehydrogenase.

Mean Serum Concentration of Immunoglobulin G (IgG)11 months

Blood samples was collected at various times after HLH diagnosis to determine the concentration of IgG.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Brigham and Women's Hospital

🇺🇸

Boston, Massachusetts, United States

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