Investigation of Relationship Between Cholesteryl Ester Transfer Protein Deficiency and Carotid/Coronary Atherosclerosis in Patients With Hyperalphalipoproteinemia
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Low CETP Activity
- Sponsor
- Osaka University
- Enrollment
- 1500
- Locations
- 1
- Primary Endpoint
- Difference of Ischemic changes of ECG among subjects with low/high CETP mass/activity and HDL-C level
- Last Updated
- 11 years ago
Overview
Brief Summary
The aim of this study is to determine whether high high density lipoprotein-cholesterol(HDL-C) level and low Cholesteryl Ester Transfer Protein(CETP) activity is atherogenic or not in subjects who received health checkups. We investigate the association between CETP activities and the severity of atherosclerosis assessed by intima-media thickness (IMT) and compare the atherogenic change between in subjects with high HDL-C level, low HDL-C level, high CETP activities and low CETP activities by examining the morbidity rate of atherogenic diseases, the rate of ischemic electrocardiography(ECG) change, Calc Score of artery from chest X-ray, Ankle Brachial Index/Pulse Wave Velocity and various serum atherogenic markers. And we also examine the correlation between normal lipid profile and concentration, activity and function of surface lipoprotein in subjects with variety of lipoprotein levels, including patients with hyper-LDL-cholesterolemia, hyper-HDL-cholesterolemia with low or no CETP activity, patients with high level of remnant cholesterol or hyperlipoproteinemia of apolipoprotein(Apo)B-48.
Investigators
Eligibility Criteria
Inclusion Criteria
- Not provided
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Difference of Ischemic changes of ECG among subjects with low/high CETP mass/activity and HDL-C level
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of Ischemic changes of ECG was assessed between in subjects with high CETP mass/activity and in those with low CETP mass/activity, and between in subjects with high HDL-C level and in those with low HDL-C level
Difference of Calc Score of Aorta by Chest X-ray among subjects with low/high CETP mass/activity and HDL-C level
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of Calc Score of Aorta by Chest X-ray was assessed between in subjects with high CETP mass/activity and in those with low CETP mass/activity, and between in subjects with high HDL-C level and in those with low HDL-C level
Difference of Ankle Brachial Index (ABI) among subjects with low/high CETP mass/activity and HDL-C level
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of Ankle Brachial Index (ABI) was assessed between in subjects with high CETP mass/activity and in those with low CETP mass/activity, and between in subjects with high HDL-C level and in those with low HDL-C level
Difference of CETP activity between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of CETP activity was assessed between in those who do not satisfy any of these three conditions (normolipidemic subjects) and those who satisfy any of these three conditions (patients with dyslipidemia); LDL-C:100 mg/dl and above, HDL:80 mg/dl and above, TG:150 mg/dl and above.
Difference of Monocyte chemoattractant protein-1 (MCP-1) concentration between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of MCP-1 concentration was assessed between normolipidemic subjects and patients with dyslipidemia
Difference of Pulse Wave Velocity (baPW) among subjects with low/high CETP mass/activity and HDL-C level
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of Pulse Wave Velocity (baPW) was assessed between in subjects with high CETP mass/activity and in those with low CETP mass/activity, and between in subjects with high HDL-C level and in those with low HDL-C level
Difference of IMT and Plaque Score of Carotid artery among subjects with low/high CETP mass/activity and HDL-C level
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of IMT and Plaque Score of Carotid artery was assessed between in subjects with high CETP mass/activity and in those with low CETP mass/activity, and between in subjects with high HDL-C level and in those with low HDL-C level
Difference of Antioxidant activity between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of Antioxidant activity was assessed between normolipidemic subjects and patients with dyslipidemia;HDL from subjects' serum samples will be added to LDL with 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH).
Difference of Cholesterol efflux between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of Cholesterol efflux by HDL from macrophage was assessed between normolipidemic subjects and patients with dyslipidemia
Difference of Proteome analysis of lipoproteins between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)
Time Frame: participants will be followed for the duration of hospital stay（2 days）
The difference of Proteome analysis of lipoproteins (HDL, LDL, remnant binding protein) was assessed between normolipidemic subjects and patients with dyslipidemia
Secondary Outcomes
- Difference of waist circumference among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of triglyceride (TG) among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Apolipoprotein (apo)B-48 concentrations among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Remnant lipoprotein (RemL-C) between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)(participants will be followed for the duration of hospital stay（2 days）)
- Difference of BMI among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Blood Pressure among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Remnant lipoprotein (RemL-C) among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Fasting blood glucose and HbA1c among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Smoking habits, alcohol taking habits and fitness habits among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Total Cholesterol(TC) among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of low density lipoprotein-cholesterol (LDL-C) among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Serum adiponectin among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of 75g glucose tolerance test and calculated homeostasis model assessment of insulin resistance (HOMA-IR) among subjects with low/high CETP mass/activity and HDL-C level(participants will be followed for the duration of hospital stay（2 days）)
- Difference of Lipoprotein Profile between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)(participants will be followed for the duration of hospital stay（2 days）)
- Difference of apolipoprotein(apo)B-48 concentrations between normolipidemic subjects and patients with dyslipidemia (including high HDL-C level)(participants will be followed for the duration of hospital stay（2 days）)