Mechanism of Aphakic and Pseudophakic Glaucoma in Pediatric Patients

Completed

• Conditions: Congenital Cataract; Aphakic or Pseudophakic Glaucoma

• Registration Number: NCT01136473
• Lead Sponsor: Carmel Medical Center

Brief Summary

In cell cultures, lens epithelial cells and trabecular meshwork cells will be exposed to aqueous humor from pediatric eyes undergoing cataract removal surgery. Cytokine analysis of candidate factors will be performed before and after exposure to lens epithelial.

Detailed Description

Aqueous from the anterior chamber will be withdrawn before surgery. Then the lens capsule will be opened (capsulorhexis) and removed. The lens material will be removed. All 3 components will be sent to the lab.

Lens epithelial cells will be isolated and will be used in a co-culture setup with trabecular cells to investigate the interactions between lens cells and trabecular cells. Understanding the interaction between young and adult lens cells on trabecular cell will shed light on mechanism of infantile aphakic glaucoma.

Aqueous humor taken out before cataract surgery and before glaucoma operation of aphakic eyes will be used for analysis of growth factor and cytokines in the Aphakic eye using proteins membrane arrays. Eyes before cataract surgery will be used as controls. Such analysis will shed light on the possible factors affecting trabecular cells in aphakic eye.

Study Timeline

• Study First Submitted: 2010-05-27
• Study First Submitted QC: 2010-06-02
• Study First Posted: 2010-06-03
• Study Start: 2011-07
• Primary Completion: 2014-09
• Study Completion: 2014-09
• Status Verified: 2015-02
• Last Update Submitted: 2015-02-22
• Last Update Posted: 2015-02-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 40

Inclusion Criteria

• children with congenital cataract and are going to do removal surgery
• children with aphakic or pseudophakic glaucoma and are going to do glaucoma surgery
• both of parents are able to sign the INFORMED CONSENT FORM

Exclusion Criteria

• patients with other eye diseases apart from congenital cataract(in children with congenital cataract) and aphakic or pseudophakic glaucoma (in children with aphakic or pseudophakic glaucoma)

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
cell culture	one year	In cell cultures, lens epithelial cells and trabecular meshwork cells will be exposed to aqueous humor from pediatric eyes undergoing cataract removal surgery. Cytokine analysis of candidate factors will be performed before and after exposure to lens epithelial.

Trial Locations

Locations (1)

Carmel Medical Center; 🇮🇱 Haifa, Israel