Confounding Factors in Ocular Surface Microbiome Research
Not Applicable
Completed
- Conditions
- Ocular Surface Microbiome
- Interventions
- Diagnostic Test: Conjunctival swab
- Registration Number
- NCT04193917
- Lead Sponsor
- Universitaire Ziekenhuizen KU Leuven
- Brief Summary
To elucidate confounding factors in ocular surface microbiome research and to confirm the presence of a core ocular surface microbiome and its temporal stability.
- Detailed Description
The investigator will take a conjunctival sample of both eyes of a group of volunteers together with an extensive questionnaire. Furthermore, 50 volunteers will be sampled repeatedly during one year or two years.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 450
Inclusion Criteria
- The subject is fluent in written and verbal Dutch
- The subject is capable of giving informed consent
Exclusion Criteria
- Use of glaucoma drops
- Use of antibiotic drops < 1 month before sampling
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description conjunctival swab Conjunctival swab A conjunctival swab of both eyes will be taken
- Primary Outcome Measures
Name Time Method Richness and evenness of distribution of the microbial flora measured as operational taxonomic units on a single moment 20 minutes for samples and filling the questionnaires A conjunctival swab and extensive questionnaire will be taken to search for a core ocular surface microbiome and confounding factors
Change in richness and evenness of distribution of the microbial flora, measured as operational taxonomic units, over time 20 minutes for the first sample and questionnaire, 5 minutes for all other visits A conjunctival swab will be taken on different points in time to investigate the temporal stability of the conjunctival microbial flora
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
University Hospitals Leuven, Department of Ophthalmology
🇧🇪Leuven, Belgium