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Interrupters of VAscular daMAge in Malignant Hypertension

Completed
Conditions
Malignant Hypertension
Interventions
Biological: analyse of angiogenic, vasoactive and VEGF systems
Registration Number
NCT04991077
Lead Sponsor
Centre Hospitalier de PAU
Brief Summary

The pathophysiology of malignant hypertension is poorly understood. The objective of this translational research project is to evaluate the relationship between activation of vasoactive systems (renin-angiotensin and endothelin systems), angiogenic signal deficiency (VEGF and sFlt-1) and the occurrence of malignant hypertension episodes in humans.

Detailed Description

The pathophysiology of malignant hypertension is poorly understood. The current dogma is based on an overwhelming renin-angiotensin-aldosterone system activation, leading to arterial hypertension that overcomes target organ auto-regulatory mechanisms and leads to subacute microvascular lesions. However, some patients present with normal or lowered renin in the acute phase of malignant hypertension, suggesting other pathophysiological pathways. Malignant hypertension was reported following anti-VEGF treatment, suggesting that this pathway may be involved. Recent unpublished animal data highlight 1/ the possibility of severe deregulation of the VEGF (vascular endothelial growth factor) system in malignant hypertension 2/ the possibility of compensation of the vasculotoxic effects of VEGF deficiency by inflammasome components. These systems have never been studied together in human hypertension.

Investigators will analyze the angiogenic, vasoactive and VEGF systems through blood and urine sampling. These samples will be collected at the time of malignant hypertension diagnosis and repeated one month later in 30 patients. The same tests will be performed in 15 patients with severe non-malignant hypertension, constituting the control group.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
45
Inclusion Criteria

Patients group :

  • Patients included in the HAMA cohort
  • Who is willing to take part in the IVAMA project

Control group :

  • Grade 2 or 3 hypertension with office blood pressure measurement (above 160 and/or 100 mmHg for systolic and diastolic)
  • Persistence of blood pressure above 160 / 100 mmHg on the average of 3 "attended" blood pressure measurements

Exclusion criteria:

Patients group :

  • Age < 18 years old
  • Patients with chronic renal failure of stage 3 or higher.
  • Patients with any type of diabetes
  • Patient in per partum
  • Patients who cannot freely give their consent, or patients who refuse to participate
  • Chronic dialysis patient

Control group:

  • Evidence of subacute involvement of one of the following target organs: brain, kidney, eye, heart, thrombotic microangiopathy. Target organ impairment is defined in the inclusion criteria for the "Patients" group.
  • Presence of known chronic kidney insufficiency of grade 3 or higher
  • Chronic dialysis patient
  • Diabetes of any type
  • Patients who cannot freely give their consent, or patients who refuse to participate
Exclusion Criteria

Not provided

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Control groupanalyse of angiogenic, vasoactive and VEGF systemspatients with severe hypertension (Grade 2 or 3 hypertension)
patients groupanalyse of angiogenic, vasoactive and VEGF systemspatients with malignant hypertension
Primary Outcome Measures
NameTimeMethod
sFLT1 concentration at inclusionat the end of study recrutment, an average of 11 month

The primary endpoint will be the difference in sFLT1 concentrations between patients and controls at enrolment

Secondary Outcome Measures
NameTimeMethod
VEGF concentration at inclusionat the end of study recrutment, an average of 11 month

Difference in VEGF concentrations between patients and controls at enrolment

renin concentration at inclusionat the end of study recrutment, an average of 11 month

Difference in renin concentrations between patients and controls at enrolmentD30, and compare this evolution.

evolution of renin concentrationthrough study completion, an average of 12 month

Evaluation of the evolution of renin concentration in the two groups between D0 and D30, and compare this evolution.

IL1ß concentration at inclusionat the end of study recrutment, an average of 11 month

Difference in IL1ß concentrations between patients and controls at enrolment

evolution of VEGF concentrationthrough study completion, an average of 12 month

Evaluation of the evolution of VEGF concentration in the two groups between D0 and D30, and compare this evolution.

evolution of angiotensin concentrationthrough study completion, an average of 12 month

Evaluation of the evolution of angiotensin concentration in the two groups between D0 and D30, and compare this evolution.

angiotensin concentration at inclusionat the end of study recrutment, an average of 11 month

Difference in angiotensin concentrations between patients and controls at enrolmentD30, and compare this evolution.

evolution of IL1ß concentrationthrough study completion, an average of 12 month

Evaluation of the evolution of IL1ß concentration in the two groups between D0 and D30, and compare this evolution.

mutations in the genes of interestthrough study completion, an average of 11 month

comparison in the 2 groups of the frequency of mutations in the genes of interest underlying the vasoactive, angiogenic and VEGF systems

Trial Locations

Locations (7)

Hôpital Avicenne

🇫🇷

Bobigny, France

CHU de Bordeaux

🇫🇷

Bordeaux, France

Hôpital Bichat

🇫🇷

Paris, France

Hôpital Européen Georges Pompidou

🇫🇷

Paris, France

Hôpital Tenon

🇫🇷

Paris, France

Chu Rangueil

🇫🇷

Toulouse, France

CHU de Tours

🇫🇷

Tours, France

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