Sequential Versus Single Step Culture Media in Relation to Euploidy Rates in PGTai Program
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 200
- 试验地点
- 2
- 主要终点
- Mosaicism rate
研究概览
简要总结
A prospective cohort study undergoing ICSI at a private fertility center in Egypt
详细描述
Purpose:
To evaluate PGTai results for embryos cultured in sequential versus single step media.
Method:
Injected oocytes will divided between Continuous Single Culture-NX media (CSC- NX) with 10% SSS the first group (Irvine, USA) and the other group will be cultured in sequential cleavage and blastocyst media with 10% SPS (SAGE, Denmark).
All blastocytes with 4BB quality and better according to Gardner's criteria 1999 will go through PGTA procedures with PGTai confirmation.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 37 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Female age ≤37 years with ≥ 8 mature oocytes
- •Male partner had normal semen parameters according to WHO 2010
- •PGT-A was done at the blastocyst stage
排除标准
- •sperm or oocyte donor
- •severe male factors according to WHO 2010
结局指标
主要结局
Mosaicism rate
时间窗: 5:7 days of ICSI
percentage of mosaic embryos/ total genetic tested embryos on blast stage
Euploidy rate
时间窗: 5:7 days of intracytoplasmic sperm injection(ICSI)
percentage of euploid embryos/ total genetic tested embryos on blast stage
次要结局
- Fertilization rate(16:19 hours of ICSI)
- High quality of cleaved embryos(72 hours of ICSI)
- Blast formation rate(5:7 days of ICSI)
- Cleavage stage(72 hours of ICSI)
- High quality blast rate(5:7 days of ICSI)