Comparison of Individual Culture and Group Culture of Preimplantation Embryos in Human IVF Treatments

Completed

• Conditions: Other Complications Associated With Artificial Fertilization
• Interventions: Other: individual embryo culture or group culture

• Registration Number: NCT01774006
• Lead Sponsor: Semmelweis University

Brief Summary

Prospective randomized comparison of two widely used in vitro culture system in human IVF treatments. In vitro fertilized oocytes and embryos are cultured in individual culture media droplets using conventional petridish or in group culture using WOW (well of the well) petridish.

Primary outcome: implantation and pregnancy rates Secondary outcome: fertilization rate and embryo quality

Detailed Description

Not available

Study Timeline

• Study Start: 2012-09
• Study First Submitted: 2013-01-18
• Study First Submitted QC: 2013-01-18
• Study First Posted: 2013-01-23
• Primary Completion: 2015-05
• Status Verified: 2015-09
• Study Completion: 2015-09
• Last Update Submitted: 2015-09-01
• Last Update Posted: 2015-09-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 532

Inclusion Criteria

• Infertile patients entering in vitro fertilization treatment
• at least 1 oocyte collected

Exclusion Criteria

• no oocytes collected

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Group culture	individual embryo culture or group culture	Embryos cultured in groups of 2-10
Individual culture	individual embryo culture or group culture	Embryos cultured individually

Primary Outcome Measures

Name	Time	Method
Clinical pregnancy rate	4 weeks after the embryo transfer	Number of clinical pregnancies (/No. of embryo transfers)
Implantation rate	4 weeks after embryo transfer	Number of embryos implanted (/number of embryos transferred)

Secondary Outcome Measures

Name	Time	Method
Embryo quality	36 and 72 hours after fertilization	Number and quality of blastomeres, amount of fragmentation embryo grade
Fertilization rate	18 hours after sperm injection	Number of oocytes fertilized. (only in intracytoplasmic sperm injection cycles)

Trial Locations

Locations (1)

Semmelweis University First Department of Obstetrics and Gynecology; 🇭🇺 Budapest, Hungary