Impact of Non Digestible Carbohydrate on Production of Phenolic Acids From Strawberry Juice

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Dietary Supplement: Strawberry Juice; Dietary Supplement: Inulin; Dietary Supplement: Strawberry juice with Inulin

• Registration Number: NCT03383809
• Lead Sponsor: University of Glasgow

Brief Summary

This is an acute human bioavailability study in self-reported healthy participants aged 20-70 years old. We hypothesize that combination of dietary polyphenolics and non-digestible carbohydrates (NDC) will increase the production of phenolic acids by bacteria in the human colon and these will be detected in urine. Participants will attend for three arms in a randomised order: Strawberry juice (a high polyphenol food), Inulin (NDC) or Mixture of strawberry juice and inulin.

Detailed Description

Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols. The colonic microbiota are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as dietary fibre to short chain fatty acids. It is likely that there are key interactions in the colonic bacteria metabolism of fibre and phenolics. We hypothesize that combination of polyphenolics and non-digestible carbohydrates (NDC) will increase the urinary output of bioactive phenolic acids.

This study will enable a better understanding of how to deliver combinations of ingredients and nutrients to achieve maximum nutritional value and health benefits.

Study Timeline

• Study Start: 2017-04-28
• Study First Submitted: 2017-12-19
• Study First Submitted QC: 2017-12-19
• Study First Posted: 2017-12-26
• Status Verified: 2019-07
• Primary Completion: 2019-07-06
• Study Completion: 2019-07-06
• Last Update Submitted: 2019-07-11
• Last Update Posted: 2019-07-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 15

Inclusion Criteria

Self-reported healthy adults

Exclusion Criteria

Antibiotic use within the last 3 months, identified gastro-intestinal diseases, on prescribed medication other than the contraceptive pill, individuals who are pregnant or breastfeeding. Individuals who have been diagnosed as anaemic, as well as those who are allergic to any food, or paracetamol.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Strawberry juice	Strawberry Juice	One dose of 300 g of strawberry juice will be given to subjects in the form of juice
Inulin	Inulin	One dose of 10 g of inulin will be given to subjects in the form of a drink
Strawberry juice with inulin	Strawberry juice with Inulin	One dose of 300 g of strawberry with 10 g of inulin will be given to subjects in the form of juice

Primary Outcome Measures

Name	Time	Method
Phenolic acids bioavailability	0-24 hrs	Urine excretion of phenolic acids

Secondary Outcome Measures

Name	Time	Method
Gastric emptying time	0-6 hrs	Plasma paracetamol levels by acetaminophen assay kits
Urolithin bioavailability	0-24 hrs	Urine excretion of urolithins
Glycaemic and appetite hormones measurements by ELISA	0-8 hrs	Plasma glucose, insulin and appetite hormone levels
Mouth to caecum transit time	0-8 hrs	Breath hydrogen level measurements by Hydrogen monitor

Trial Locations

Locations (1)

School of Medicine, Nursing and Dentistry, College of MVLS, University of Glasgow; 🇬🇧 Glasgow, Lanarkshire, United Kingdom