Asprosin Level in Individuals with Periodontal Disease

Completed

• Conditions: Periodontitis; Periodontal Health; Gingivitis

• Registration Number: NCT06627972
• Lead Sponsor: Mustafa Kemal University

Brief Summary

The aim of this study was to investigate the levels of asprosin hormone and tumour necrosis factor alpha (TNF-α) in gingival crevicular fluids and to compare its level with those in saliva and serum in patients with gingivitis and periodontitis.

Detailed Description

This study is investigating the level of asprosin hormone in gingival crevicular fluid of patients with periodontal diseases such as gingivitis and periodontitis, and observing the healthy patient in terms of the hormone levels, as well. 75 volunteer patients aged 18-45 years old were included in this study. These patients were divided equally to three groups: Group I; periodontal health, Group II; gingivitis, Group III; periodontitis.The body fluids including serum, saliva and gingival crevicular fluid were collected from this participants according to the proper protocol and their storage was provided under certain conditions. ELISA tests for asprosin and Tumour necrosis factor alpha were made in laboratory. Also, clinical measurements such as plaque index, bleeding on probing, probing pocket depth were recorded at first visit day of the participants. The data were analysed according to the different periodontal conditions, and correlated with asprosin hormone and tumour necrosis factor alpha level in gingival crevicular fluid, serum and saliva.

Study Timeline

• Study Start: 2022-01-01
• Primary Completion: 2022-07-31
• Study Completion: 2022-08-31
• Status Verified: 2024-10
• Study First Submitted: 2024-10-01
• Study First Submitted QC: 2024-10-03
• Last Update Submitted: 2024-10-03
• Study First Posted: 2024-10-04
• Last Update Posted: 2024-10-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 75

Inclusion Criteria

Not provided

Exclusion Criteria

• Those with any systemic disease
• Individuals who take medication regularly
• Pregnant or lactating women
• Individuals with menopause and anthropause
• Those who have undergone any periodontal treatment in the last six months
• Smokers

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Asprosin level	Day 1	Asprosin level (ng/mL) in gingival crevicular fluid, serum and saliva.

Secondary Outcome Measures

Name	Time	Method
TNF-alpha levels	Day 1	TNF-alpha level (pg/mL) in gingival crevicular fluid, serum and saliva
Plaque index	Day 1	The index was recorded from all participants as numerical between 0-3
bleeding on probing	Day 1	The bleeding on probing is stated by percentage. the ratio of the number of sites with bleeding gingiva on probing around teeth to total surfaces of all examined teeth
probing pocket depth	Day 1	The parameters were recorded as mm in all participant. The probing pocket depth is measured from the marginal gingiva to bottom of pocket using a periodontal probe, from six surfaces of each tooth.
body mass index	Day 1	the weight and height is combined to report BMI in kg/m2

Trial Locations

Locations (1)

Adiyaman University, Faculty of Dentistry; 🇹🇷 Adıyaman, Turkey