Vascular Response to Isoproterenol and β2 Adrenergic Receptor Polymorphisms

Not Applicable

Completed

• Conditions: Coronary Disease

• Registration Number: NCT00226551
• Lead Sponsor: Hadassah Medical Organization

Brief Summary

Single nucleotide polymorphisms at codon 46 and 79 of the gene encoding for the ß2 adrenergic receptor (ß2AR) modify its pharmacological properties and may alter the response to ß2AR agonists. The goal of the present study was to evaluate the role played by the Arg16Gly and Gln27Glu polymorphisms on isoproterenol induced relaxation of internal mammary arteries segments ex-vivo.

Internal mammary leftover segments were collected from 96 patients undergoing coronary artery bypass graft operation. Four rings that were prepared from each specimen were allowed to reach equilibrium with physiological Krebs solution prior to precontraction with U46619. Using the organ bath technique, cumulative dose response curve of isoproterenol was constructed and mean EC50 calculated for each patient.

Detailed Description

Not available

Study Timeline

• Study Start: 1999-08
• Study First Submitted: 2005-09-12
• Study First Submitted QC: 2005-09-25
• Study First Posted: 2005-09-27
• Status Verified: 2008-10
• Last Update Submitted: 2008-10-28
• Last Update Posted: 2008-10-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• patients scheduled to undergo coronary artery bypass graft operation

Exclusion Criteria

• Chronic treatment with corticosteroids

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Primary Outcome Measures

Name	Time	Method
Mean Ec50% in response to rising concentration of isoproterenol

Trial Locations

Locations (1)

Hadassah Medical Organization; 🇮🇱 Jerusalem, Israel