In Vitro Maturation Induction of Oocytes From a Woman With Syndrome of Repeated Oocyte Maturation Failure in Vitro

Completed

• Conditions: Oocyte Maturation Failure
• Interventions: Other: no intervention

• Registration Number: NCT01977664
• Lead Sponsor: The Second Affiliated Hospital of Kunming Medical University

Brief Summary

Donated oocytes is from a patient with repeated oocyte maturation failure in IVF cycles. Cytoplasm of matured metaphase II oocyte and active MPF were injected into the donated oocytes.

Detailed Description

The study reported a woman with syndrome of repeated oocyte maturation failure where there is repeated production of immature oocytes.She underwent two IVF cycles in other hospital. All oocytes obtained were immature and the treatment were stopped.She underwent her third try in our hospital. The couple gave their informed consent: ICSI will be performed if maturation occurred, otherwise, the treatment would be stopped and the oocytes would be donated for study if the oocytes were not matured after 24 hours of culture. After ovarian hyperstimulation,14 oocytes were obtained. None of them was matured. The cycle was canceled and the oocytes were donated for study.To induce the immature oocytes in vitro, oocytes were 1)cultured in follicular fluid containing matured metaphase II oocyte; 2) performed intracytoplasmic sperm injection (ICSI) to induce intracellular Ca2+\]i oscillations; 3)injected with active maturation promoting factor (MPF), 4)injected with cytoplasm of matured metaphase II oocyte.

Study Timeline

• Study Start: 2012-03
• Primary Completion: 2012-04
• Study Completion: 2012-04
• Status Verified: 2013-10
• Study First Submitted: 2013-10-09
• Study First Submitted QC: 2013-11-06
• Last Update Submitted: 2013-11-06
• Study First Posted: 2013-11-07
• Last Update Posted: 2013-11-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 1

Inclusion Criteria

Clinical diagnosis of syndrome of repeated oocyte maturation

Exclusion Criteria

patients with matured oocytes in IVF cycles

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
oocyte maturation failure	no intervention	no intervention

Primary Outcome Measures

Name	Time	Method
Oocyte nucleus maturation	April，2012

Trial Locations

Locations (1)

The Second Affiliated Hospital of Kunming Medical University; 🇨🇳 Kunming, Yunnan, China