Characterization of Exosomes Platelets-released
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Platelet Thrombus
- Sponsor
- Centro Cardiologico Monzino
- Enrollment
- 175
- Locations
- 1
- Primary Endpoint
- Characterization of exosomes released by activated platelet by Nanoparticle Tracking Analysis (NTA) and proteoma analysis
- Status
- Recruiting
- Last Updated
- 2 years ago
Overview
Brief Summary
Extracellular vesicles (EVs) play a key role in cell-to-cell communication. They are small vesicles that contain rich molecular cargo. Recently, they have been proposed as biomarkers for clinical diagnostics. EVs include three classes: small EVs (exosomes), large EVs (microparticles), and apoptotic bodies. Platelet-derived EVs (PEVs) are the most abundant class in human blood and can actively participate in numerous physiological and pathological processes. The information about the role of platelet exosomes in cardiovascular disease and the effect of antiplatelet agents on their release and content is very limited.
Detailed Description
This study is a multicentre, observational. We plan to enrol 54 subjects from Centro Cardiologico Monzino IRCCS, and 121 buffy coat related to 121 subjects from Centro Trasfusionale Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico. biographical and anthropomorphic data will be recorded, a fasting blood venous sample (from ante-cubital vein) will be collected for the haematochemical analyses and for research samples.
Investigators
Eligibility Criteria
Inclusion Criteria
- •subjects without known pathologies
Exclusion Criteria
- •individuals with gastrointestinal diseases,
- •individuals with history of hepatic diseases,
- •individuals with history of urogenital diseases,
- •individuals with history of hematological diseases,
- •individuals with history of immunological diseases,
- •individuals with history of renal diseases,
- •individuals with history of metabolic diseases
- •individuals with history of respiratory diseases
- •individuals with history of cancer
- •individuals with history of cardiovascular disease
Outcomes
Primary Outcomes
Characterization of exosomes released by activated platelet by Nanoparticle Tracking Analysis (NTA) and proteoma analysis
Time Frame: 36 month
Characterization of exosomes released by activated platelet and exposed to platelet inhibitors by NTA and proteoma analysis
Time Frame: 36 month
Secondary Outcomes
- Platelet exosome profile emerged by NTA and proteoma analysis will be related with the "biographical" and "anthropometric" data of the subjects.(40 month)