The function and composition of B cells in patients with Glioblastoma treated with and without Dexamethasone

Completed

Conditions: Brain Tumor
Glioblastoma
10029211

Registration Number: NL-OMON49155

Lead Sponsor: Vrije Universiteit Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 20

Inclusion Criteria

1. MRI scan and patient history highly suspicious for a high grade
glioma/glioblastoma
2. Indication for surgery as determined by the Multidisciplinairy Brain tumor
Board
3. Patients are 18 years or older at first diagnosis
4. Patients are willing and able to give written informed consent

Exclusion Criteria

1. No indication for surgery to confirm radiological diagnosis
2. Not able or willing to give informed consent
3. Allergy or intolerance to dexamethasone
4. Diagnosis of glioblastoma could not be confirmed by histological examination

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>In order to quantify and characterize atypic B cells, we have designed a B cell<br /><br>focused panel for analysis by means of mass cytometry that includes the<br /><br>following markers: CD45, CD49d, CD19, CD5, CCR5, CD7, IgD, CD20, IgA, CD25,<br /><br>CD86, CD123, CD21, CD45RA, CD14, CD27, CXCR3, CCR4, CD22, CTLA-4, CD79b,<br /><br>CD95Fas, CD45RO, CD44, CD38, CD73, CD24, CD3, CD9, IgM, HLA-DR, CD71, CD127,<br /><br>and CD16. Data acquisition and analysis will proceed as previously reported<br /><br>(Dusoswa et al., 2019). In addition to this, we will use a smaller panel<br /><br>containing a selection of the markers above with the addition of intracellular<br /><br>IL-10, as reported by others (Wiest et al., 2019).</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>We will collect information regarding potential confounding factors, such as<br /><br>tumor volume, age, body weight, smoking, and other co-morbidities.</p><br>