Effects of Ozone Therapy Modalities on Biochemical Markers of Muscle Damage Following Eccentric Exercise
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 32
- 试验地点
- 1
- 主要终点
- CK
研究概览
简要总结
The goal of this clinical trial is to determine the effectiveness of different ozone therapy methods in enhancing recovery after muscle-damaging exercise in healthy adults, and to evaluate their impact on biochemical markers of muscle damage and fatigue.
The main questions it aims to answer are:
- Do ozone-based interventions accelerate recovery by reducing biochemical markers of muscle damage and fatigue following eccentric exercise?
- Which ozone therapy modality provides the greatest recovery benefit? Researchers will compare three intervention groups (autohemotherapy, ozone sauna therapy, and ozonated lavender oil massage) with a control group receiving no treatment.
Participants will:
- Complete a standardized eccentric exercise protocol designed to induce muscle damage
- Receive one of the assigned interventions or no treatment (control)
- Provide blood samples at multiple time points (baseline, post-exercise, post-intervention, and 24, 48, and 72 hours follow-up) to assess recovery- related biomarkers
详细描述
This randomized controlled trial was designed to investigate the effects of different ozone therapy modalities on recovery following eccentric exercise-induced muscle damage in healthy male amateur collegiate football players aged 18-30 years.
Thirty-two participants were randomly assigned to one of four groups: autohemotherapy, ozone sauna therapy, ozonated lavender oil massage, or a no-intervention control group (n = 8 per group). All participants completed a standardized eccentric exercise protocol to induce muscle damage and fatigue.
Following exercise, participants in the intervention groups received a single session of their assigned treatment, while the control group remained at rest without any recovery intervention. Autohemotherapy involved withdrawal of venous blood, exposure to an oxygen-ozone mixture, and reinfusion. Ozone sauna therapy was applied in a sealed cabin under controlled conditions with measures to prevent inhalation. Ozonated lavender oil massage was administered to the lower extremities using standardized massage techniques.
Blood samples were collected at baseline, immediately post-exercise, post-intervention, and at 24, 48, and 72 hours after intervention. Serum levels of lactate dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and uric acid (UA) were analyzed as indicators of muscle damage and metabolic stress.
Statistical analysis was performed using two-way permutational multivariate analysis of variance (PERMANOVA) to assess group, time, and group × time interaction effects.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •male football players aged 18-30 years
- •playing exclusively in the midfield position
- •active participation in regional professional football leagues (non-top-tier)
- •engagement in regular football training at least four sessions per week with a minimum duration of 90 minutes per session
- •absence of acute or chronic musculoskeletal injuries within the preceding six months
- •no use of anti-inflammatory medications or ergogenic supplements within the four weeks prior to the study
排除标准
- •female football players
- •diagnosed cardiovascular, metabolic, or neurological disorders
- •known hypersensitivity to ozone exposure or lavender oil
- •any surgical intervention within the past six months
- •concurrent participation in alternative recovery or therapeutic interventions during the study period
- •playing positions other than midfield
结局指标
主要结局
CK
时间窗: Baseline, immediately post-exercise, immediately after ozone sauna treatment, 24 hours after ozone sauna treatment, 48 hours after ozone sauna treatment and 72 hours after ozone sauna treatment.
Serum Creatine Kinase level
次要结局
- LDH(Baseline, immediately post-exercise, immediately after ozone sauna treatment, 24 hours after ozone sauna treatment, 48 hours after ozone sauna treatment and 72 hours after ozone sauna treatment.)
- AST(Baseline, immediately post-exercise, immediately after ozone sauna treatment, 24 hours after ozone sauna treatment, 48 hours after ozone sauna treatment and 72 hours after ozone sauna treatment.)
- UA(Baseline, immediately post-exercise, immediately after ozone sauna treatment, 24 hours after ozone sauna treatment, 48 hours after ozone sauna treatment and 72 hours after ozone sauna treatment.)
- ALT(Baseline, immediately post-exercise, immediately after ozone sauna treatment, 24 hours after ozone sauna treatment, 48 hours after ozone sauna treatment and 72 hours after ozone sauna treatment.)
研究者
SERKAN DÜZ
Principal Investigator
Inonu University